{"entity": "journal", "iuid": "c47b62c805e64fdbb8b93b132e5ea4fd", "timestamp": "2026-07-11T06:13:38.002Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/J%20Glob%20Antimicrob%20Resist.json"}, "display": {"href": "https://publications.scilifelab.se/journal/J%20Glob%20Antimicrob%20Resist"}}, "title": "J Glob Antimicrob Resist", "issn": "2213-7173", "issn-l": "2213-7165", "publications_count": 2, "publications": [{"entity": "publication", "iuid": "e60dd6b645a64a3994590cd483f7d3ef", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e60dd6b645a64a3994590cd483f7d3ef.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e60dd6b645a64a3994590cd483f7d3ef"}}, "title": "Identification of novel FosX family determinants from diverse environmental samples.", "authors": [{"family": "Kieffer", "given": "Nicolas", "initials": "N"}, {"family": "B\u00f6hm", "given": "Maria-Elisabeth", "initials": "ME"}, {"family": "Berglund", "given": "Fanny", "initials": "F"}, {"family": "Marathe", "given": "Nachiket P", "initials": "NP"}, {"family": "Gillings", "given": "Michael R", "initials": "MR"}, {"family": "Larsson", "given": "D G Joakim", "initials": "DGJ"}], "type": "journal article", "published": "2024-12-24", "journal": {"title": "J Glob Antimicrob Resist", "issn": "2213-7173", "volume": "41", "pages": "8-14", "issn-l": "2213-7165"}, "abstract": "This study aimed to identify novel fosfomycin resistance genes across diverse environmental samples, ranging in levels of anthropogenic pollution. We focused on fosfomycin resistance, and given its increasing clinical importance, explored the prevalence of these genes within different environmental contexts.\n\nMetagenomic DNA was extracted from wastewater and sediment samples collected from sites in India, Sweden, and Antarctica. Class 1 integron gene cassette libraries were prepared, and resistant clones were selected on fosfomycin-supplemented media. Long-read sequencing was performed followed by bioinformatics analysis to identify novel fosfomycin resistance genes. The genes were cloned and functionally characterized in E. coli, and the impact of phosphonoformate on the enzymes was assessed.\n\nFour novel fosfomycin resistance genes were identified. Phylogenetic analysis placed these genes within the FosX family, a group of metalloenzymes that hydrolyse fosfomycin without thiol conjugation. The genes were subsequently renamed fosE2, fosI2, fosI3, and fosP. Functional assays confirmed that these genes conferred resistance to fosfomycin in E. coli, with MIC ranging from 32 \u03bcg/ml to 256 \u03bcg/ml. Unlike FosA/B enzymes, these FosX-like proteins were resistant to phosphonoformate inhibitory action. A fosI3 homolog was identified in Pseudomonas aeruginosa, highlighting potential clinical relevance.\n\nThis study expands the understanding of fosfomycin resistance by identifying new FosX family members across diverse environments. The lack of phosphonoformate inhibition underscores the clinical importance of these poorly studied enzymes, which warrant further investigation, particularly in pathogenic contexts.", "doi": "10.1016/j.jgar.2024.12.018", "pmid": "39725324", "labels": {"NGI Uppsala (Uppsala Genome Center)": "Service", "NGI Long read": "Service", "National Genomics Infrastructure": "Service"}, "xrefs": [{"db": "pii", "key": "S2213-7165(24)00486-7"}], "notes": [], "created": "2025-03-07T10:01:40.876Z", "modified": "2025-03-07T10:01:40.879Z"}, {"entity": "publication", "iuid": "1d494d4aae3148d6adc0f0e34b6fea33", "links": {"self": {"href": "https://publications.scilifelab.se/publication/1d494d4aae3148d6adc0f0e34b6fea33.json"}, "display": {"href": "https://publications.scilifelab.se/publication/1d494d4aae3148d6adc0f0e34b6fea33"}}, "title": "A link between the newly described colistin resistance gene mcr-9 and clinical Enterobacteriaceae isolates carrying blaSHV-12 from horses in Sweden.", "authors": [{"family": "B\u00f6rjesson", "given": "Stefan", "initials": "S", "orcid": "0000-0003-2219-2659", "researcher": {"href": "https://publications.scilifelab.se/researcher/e98c1d640f9a4eeaba4563eeec9e7dff.json"}}, {"family": "Greko", "given": "Christina", "initials": "C"}, {"family": "Myren\u00e5s", "given": "Mattias", "initials": "M"}, {"family": "Land\u00e9n", "given": "Annica", "initials": "A"}, {"family": "Nilsson", "given": "Oskar", "initials": "O"}, {"family": "Pedersen", "given": "Karl", "initials": "K"}], "type": "journal article", "published": "2020-03-00", "journal": {"title": "J Glob Antimicrob Resist", "issn": "2213-7173", "volume": "20", "issue": null, "pages": "285-289", "issn-l": "2213-7165"}, "abstract": "The aim of this study was to investigate the occurrence of the newly described transferable colistin resistance gene mcr-9 in extended-spectrum \u03b2-lactamase (ESBL)-producing clinical Enterobacteriaceae isolates from horses in Sweden.\n\nA total of 56 whole-genome sequenced ESBL-producing Enterobacteriaceae isolates from horses were subjected to in silico detection of antimicrobial resistance genes and identification of plasmid replicons types. The colistin minimum inhibitory concentration (MIC) for mcr-positive isolates was determined by broth microdilution. Relatedness between Enterobacteriaceae carrying mcr genes was determined by multilocus sequence typing (MLST) and core genome MLST.\n\nThirty ESBL-producing Enterobacteriaceae isolates from horses were positive for the colistin resistance gene mcr-9. These isolates included Enterobacter cloacae, Escherichia coli, Klebsiella oxytoca and Citrobacter freundii and belonged to diverse MLST sequence types within each species. Two of the mcr-9-containing isolates originated from the same horse. All mcr-9-positive isolates had colistin MICs below or equal to the EUCAST epidemiological cut-off value of 2 mg/L and were negative for the two potential regulatory genes qseB-like and qseC-like for mcr-9. Except for one isolate carrying only blaTEM-1B, all of the isolates carried blaSHV-12 and blaTEM-1B, and were all considered multidrug-resistant as they harboured genes encoding resistance to aminoglycosides, chloramphenicol, fosfomycin, macrolides, quinolones, sulfonamides, trimethoprim and tetracyclines. Plasmid replicon types IncHI2 and IncHI2A were detected in all mcr-9-positive isolates.\n\nThe occurrence of mcr-9 was common among clinical ESBL-producing Enterobacteriaceae isolates from horses in Sweden and was linked to the ESBL-encoding gene blaSHV-12 and plasmid replicon types IncHI2 and IncHI2A.", "doi": "10.1016/j.jgar.2019.08.007", "pmid": "31494305", "labels": {"Clinical Genomics Stockholm": "Service", "Bioinformatics Support for Computational Resources": "Service", "Clinical Genomics": "Service"}, "xrefs": [{"db": "pii", "key": "S2213-7165(19)30205-X"}], "notes": [], "created": "2020-11-22T14:28:37.132Z", "modified": "2024-01-16T13:48:42.885Z"}], "created": "2020-11-22T14:28:37.142Z", "modified": "2020-11-27T13:14:09.615Z"}