{"entity": "journal", "iuid": "33bffa7a2f174bcf89f771455ab7f578", "timestamp": "2026-07-20T22:24:57.952Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/J%20Clin%20Periodontol.json"}, "display": {"href": "https://publications.scilifelab.se/journal/J%20Clin%20Periodontol"}}, "title": "J Clin Periodontol", "issn": "1600-051X", "issn-l": null, "publications_count": 3, "publications": [{"entity": "publication", "iuid": "83d4b61b754148e09946bb0b8da9c20c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/83d4b61b754148e09946bb0b8da9c20c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/83d4b61b754148e09946bb0b8da9c20c"}}, "title": "Characterising the Periodontal Granulation Tissue Using scRNAseq.", "authors": [{"family": "Zhu", "given": "Wentao", "initials": "W"}, {"family": "Fung", "given": "Kathy", "initials": "K"}, {"family": "Dhami", "given": "Pawan", "initials": "P"}, {"family": "Sharpe", "given": "Paul", "initials": "P"}, {"family": "Krivanek", "given": "Jan", "initials": "J"}, {"family": "Nibali", "given": "Luigi", "initials": "L", "orcid": "0000-0002-7750-5010", "researcher": {"href": "https://publications.scilifelab.se/researcher/aea99b6f369f449faae6eb967710ad95.json"}}, {"family": "Zhang", "given": "Cheng", "initials": "C"}, {"family": "Neves", "given": "Vitor C M", "initials": "VCM", "orcid": "0000-0002-5111-269X", "researcher": {"href": "https://publications.scilifelab.se/researcher/cd9b74ed39e340a09892f6e48690254b.json"}}], "type": "journal article", "published": "2025-10-07", "journal": {"title": "J Clin Periodontol", "issn": "1600-051X", "issn-l": null}, "abstract": "To investigate the cellular composition and molecular mechanisms of periodontal granulation tissue formation using single-cell RNA sequencing (scRNA-seq), aiming to enhance the understanding of periodontal disease pathogenesis and identify potential targets for regenerative therapies.\n\nGranulation tissue samples were collected from patients undergoing periodontal surgery (n = 3). Fresh tissues were processed into single-cell suspensions and subjected to scRNA-seq. The data were integrated and compared with existing datasets from healthy gingiva and periodontal ligament. Computational analyses were performed and validated through immunofluorescence staining.\n\nTen distinct cell clusters were identified across the samples. Granulation tissue exhibited a higher abundance of immune cells compared to healthy tissues. A novel endothelial cell subpopulation, exclusive to granulation tissue, was discovered and characterised by NOTCH3 expression and involvement in ossification pathways. Additionally, granulation tissue fibroblast subpopulations demonstrated a progenitor-like state, characterised by extracellular matrix reorganisation and low differentiation, similar to cancer-associated fibroblasts.\n\nThis study identified a novel endothelial subpopulation offering new insights into the disease's pathogenesis and presenting potential targets for regenerative therapies. These findings will help advance the understanding of periodontal disease granulation tissue formation and provide information for the development of materials to modulate specific cellular pathways to improve periodontal disease management.", "doi": "10.1111/jcpe.70048", "pmid": "41055332", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [], "notes": [], "created": "2025-11-28T10:51:16.664Z", "modified": "2025-11-28T10:51:16.748Z"}, {"entity": "publication", "iuid": "356e8dca4cb04bc6acfea23fc905db8d", "links": {"self": {"href": "https://publications.scilifelab.se/publication/356e8dca4cb04bc6acfea23fc905db8d.json"}, "display": {"href": "https://publications.scilifelab.se/publication/356e8dca4cb04bc6acfea23fc905db8d"}}, "title": "LIGHT protein: A novel gingival crevicular fluid biomarker associated with increased probing depth after periodontal surgery.", "authors": [{"family": "Esberg", "given": "Anders", "initials": "A", "orcid": "0000-0002-4430-8125", "researcher": {"href": "https://publications.scilifelab.se/researcher/a6610f154ad749a49a8f5d89382c6b1b.json"}}, {"family": "Kindstedt", "given": "Elin", "initials": "E"}, {"family": "Isehed", "given": "Catrine", "initials": "C", "orcid": "0000-0002-5327-8028", "researcher": {"href": "https://publications.scilifelab.se/researcher/6687a4367cfb42c6897083d233be0ca9.json"}}, {"family": "Lindquist", "given": "Susanne", "initials": "S"}, {"family": "Holmlund", "given": "Anders", "initials": "A"}, {"family": "Lundberg", "given": "Pernilla", "initials": "P", "orcid": "0000-0002-8244-5200", "researcher": {"href": "https://publications.scilifelab.se/researcher/e52f34dbba66471c953a8a688b33181a.json"}}], "type": "journal article", "published": "2024-07-00", "journal": {"title": "J Clin Periodontol", "issn": "1600-051X", "volume": "51", "issue": "7", "pages": "852-862", "issn-l": null}, "abstract": "To evaluate the protein profiles in gingival crevicular fluid (GCF) in relation to clinical outcomes after periodontal surgery and examine if any selected proteins affect the mRNA expression of pro-inflammatory cytokines in human gingival fibroblasts.\n\nThis exploratory study included 21 consecutive patients with periodontitis. GCF was collected, and the protein pattern (n = 92) and clinical parameters were evaluated prior to surgery and 3, 6 and 12 months after surgery. Fibroblastic gene expression was analysed by real-time quantitative polymerase chain reaction.\n\nSurgical treatment reduced periodontal pocket depth (PPD) and changed the GCF protein pattern. Twelve months after surgery, 17% of the pockets showed an increase in PPD. Levels of a number of proteins in the GCF decreased after surgical treatment but increased with early signs of tissue destruction, with LIGHT being one of the proteins that showed the strongest association. Furthermore, LIGHT up-regulated the mRNA expression of pro-inflammatory cytokines interleukin (IL)-6, IL-8 and MMP9 in human gingival fibroblasts.\n\nLIGHT can potentially detect subjects at high risk of periodontitis recurrence after surgical treatment. Moreover, LIGHT induces the expression of inflammatory cytokines and tissue-degrading enzymes in gingival fibroblasts.", "doi": "10.1111/jcpe.13964", "pmid": "38390754", "labels": {"Affinity Proteomics Uppsala": "Service"}, "xrefs": [], "notes": [], "created": "2024-11-27T22:25:23.068Z", "modified": "2024-11-27T22:25:23.165Z"}, {"entity": "publication", "iuid": "865546bfe4f74204bd6971bf9b7f056b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/865546bfe4f74204bd6971bf9b7f056b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/865546bfe4f74204bd6971bf9b7f056b"}}, "title": "Surface decontamination of explanted peri-implantitis-affected implants.", "authors": [{"family": "Ichioka", "given": "Yuki", "initials": "Y", "orcid": "0000-0003-0318-3167", "researcher": {"href": "https://publications.scilifelab.se/researcher/6de60717a790425a9304a30b8e2bc34e.json"}}, {"family": "Derks", "given": "Jan", "initials": "J", "orcid": "0000-0002-1133-6074", "researcher": {"href": "https://publications.scilifelab.se/researcher/459abeceb7c24480af9f0a444f3edd81.json"}}, {"family": "Larsson", "given": "Lena", "initials": "L"}, {"family": "Berglundh", "given": "Tord", "initials": "T", "orcid": "0000-0001-5864-6398", "researcher": {"href": "https://publications.scilifelab.se/researcher/2d29bd79b2914b47b00a0c1005b30b43.json"}}], "type": "journal article", "published": "2023-08-00", "journal": {"title": "J Clin Periodontol", "issn": "1600-051X", "volume": "50", "issue": "8", "pages": "1113-1122", "issn-l": null}, "abstract": "The present study aimed at evaluating the effect of air-polishing (AP) and a combination of AP and alkaline electrolysed water (AEW) in surface decontamination of explanted peri-implantitis-affected implants.\n\nTwenty-five patients with 34 dental implants scheduled for explantation due to severe peri-implantitis were included. Following implant removal, the apical part of each implant was embedded in acrylic blocks. Implants were randomly allocated to surface decontamination using AP with or without AEW. Four implants were left untreated and used as negative controls. Specimens were analysed using scanning electron microscopy (SEM) and energy-dispersive x-ray spectroscopy (EDS). Area of residual bacteria was the primary outcome.\n\nSEM analysis revealed that both treatment protocols were effective in biofilm removal and only small proportions of target areas of the implants showed residual bacterial or mineralized deposits. Although differences between the treatment protocols were small, implant thread loci (top/flank/valley), zones of the implant (apical/middle/coronal), implant surface characteristics and gender influenced the results. In addition, EDS analysis showed that zones influenced the atomic% of carbon and calcium and that implant surface characteristics affected the atomic% of titanium.\n\nAP, with or without AEW, is an effective method in removing biofilm from peri-implantitis-affected implants.", "doi": "10.1111/jcpe.13836", "pmid": "37271864", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [], "notes": [], "created": "2023-12-01T10:45:46.555Z", "modified": "2023-12-01T10:45:46.590Z"}], "created": "2023-12-01T10:45:46.580Z", "modified": "2023-12-01T10:45:46.580Z"}