{"entity": "journal", "iuid": "164857e1702f433d9744a7d4ffbc1508", "timestamp": "2026-07-19T18:55:43.220Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Front%20Physiol.json"}, "display": {"href": "https://publications.scilifelab.se/journal/Front%20Physiol"}}, "title": "Front Physiol", "issn": "1664-042X", "issn-l": null, "publications_count": 3, "publications": [{"entity": "publication", "iuid": "146f3a2d16da404ab95fbe8aefbc0421", "links": {"self": {"href": "https://publications.scilifelab.se/publication/146f3a2d16da404ab95fbe8aefbc0421.json"}, "display": {"href": "https://publications.scilifelab.se/publication/146f3a2d16da404ab95fbe8aefbc0421"}}, "title": "Lymphoscintigraphic alterations in lower limbs in women with lipedema in comparison to women with overweight/obesity.", "authors": [{"family": "Chachaj", "given": "Angelika", "initials": "A"}, {"family": "Dudka", "given": "Ilona", "initials": "I"}, {"family": "Jeziorek", "given": "Ma\u0142gorzata", "initials": "M"}, {"family": "Sowicz", "given": "Monika", "initials": "M"}, {"family": "Adaszy\u0144ska", "given": "Agnieszka", "initials": "A"}, {"family": "Szuba", "given": "Andrzej", "initials": "A"}], "type": "journal article", "published": "2023-04-10", "journal": {"title": "Front Physiol", "issn": "1664-042X", "volume": "14", "pages": "1099555", "issn-l": null}, "abstract": "Introduction: Lipedema is a bilateral enlargement of the legs due to abnormal depositions of subcutaneous fat. Recent studies using lymphoscintigraphy documented that lipedema associates with lymphatic alterations. It is still not known, whether non-lipedema obesity also leads to similar lymphoscintigraphic changes within lower legs. Clinically, both, lipedema and obesity may progress to secondary lymphedema. The aim of the study was to evaluate lymphoscintigraphy of lower limbs in women with lipedema in comparison to overweight/obese women. Methods: 51 women (in the mean age of 43.3 \u00b1 13.56) with the diagnosis of lipedema and 31 women (in the mean age of 44.7 \u00b1 13.48) with overweight/obesity were enrolled into the study. Women in both study groups had no clinical signs of lymphedema. The groups were matched by mean volume of their legs, calculated using the formula for a truncated cone. Lymphoscintigraphy was evaluated in every women qualitatively. Body composition parameters were assessed using bioelectric impedance analysis (BIA). Results: Lymphoscintigraphic alterations within lower extremities were similar in both, lipedema and overweight/obese groups and were present in majority of women in both study groups. The most common lymphoscintigraphic alteration in both groups were additional lymphatic vessels (in the lipedema group observed in 76.5% of patients and in the overweight/obesity group - in 93.5%). Visualization of popliteal lymph nodes and dermal backflow were observed respectively in 33% and in 5.9% in the group with lipedema and in 45.2% and in 9.7% in the overweight/obesity group. There were significant relationships between severity of lymphoscintigraphic alterations and weight, lean body mass (LBM), total body water (TBW), volume of both legs and thigh circumference in the lipedema group. Such relationships were absent in the overweight/obesity group. Discussion: Our study indicates that lymphatic alterations are present before development to clinically visible secondary lymphedema in both conditions, lipedema and overweight/obesity. In majority of women from both study groups they indicate rather an overload of the lymphatic system than insufficiency. Lymphoscintigraphic alterations are similar in both groups, therefore, lymphoscintigraphy is not a diagnostic tool that might distinguish lipedema from overweight/obesity.", "doi": "10.3389/fphys.2023.1099555", "pmid": "37101701", "labels": {"Swedish NMR Centre": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC10123278"}, {"db": "pii", "key": "1099555"}], "notes": [], "created": "2023-05-25T17:05:47.425Z", "modified": "2025-10-17T13:03:54.006Z"}, {"entity": "publication", "iuid": "52150b6016de4b65acd14618c235f5b4", "links": {"self": {"href": "https://publications.scilifelab.se/publication/52150b6016de4b65acd14618c235f5b4.json"}, "display": {"href": "https://publications.scilifelab.se/publication/52150b6016de4b65acd14618c235f5b4"}}, "title": "Spatial Statistics for Understanding Tissue Organization.", "authors": [{"family": "Behanova", "given": "Andrea", "initials": "A"}, {"family": "Klemm", "given": "Anna", "initials": "A"}, {"family": "W\u00e4hlby", "given": "Carolina", "initials": "C"}], "type": "journal article", "published": "2022-01-28", "journal": {"title": "Front Physiol", "issn": "1664-042X", "issn-l": null, "volume": "13", "issue": null, "pages": "832417"}, "abstract": "Interpreting tissue architecture plays an important role in gaining a better understanding of healthy tissue development and disease. Novel molecular detection and imaging techniques make it possible to locate many different types of objects, such as cells and/or mRNAs, and map their location across the tissue space. In this review, we present several methods that provide quantification and statistical verification of observed patterns in the tissue architecture. We categorize these methods into three main groups: Spatial statistics on a single type of object, two types of objects, and multiple types of objects. We discuss the methods in relation to four hypotheses regarding the methods' capability to distinguish random and non-random distributions of objects across a tissue sample, and present a number of openly available tools where these methods are provided. We also discuss other spatial statistics methods compatible with other types of input data.", "doi": "10.3389/fphys.2022.832417", "pmid": "35153840", "labels": {"BioImage Informatics": "Technology development", "Bioinformatics (NBIS)": "Technology development"}, "xrefs": [{"db": "pmc", "key": "PMC8837270"}], "notes": [], "created": "2022-11-02T06:13:49.813Z", "modified": "2022-11-02T06:21:28.498Z"}, {"entity": "publication", "iuid": "3c57a24495f04ec3b098f70505ca3426", "links": {"self": {"href": "https://publications.scilifelab.se/publication/3c57a24495f04ec3b098f70505ca3426.json"}, "display": {"href": "https://publications.scilifelab.se/publication/3c57a24495f04ec3b098f70505ca3426"}}, "title": "Fine Tuning of Phosphorothioate Inclusion in 2'-O-Methyl Oligonucleotides Contributes to Specific Cell Targeting for Splice-Switching Modulation.", "authors": [{"family": "Aoki", "given": "Yoshitsugu", "initials": "Y"}, {"family": "Rocha", "given": "Cristina S J", "initials": "CSJ"}, {"family": "Lehto", "given": "Taavi", "initials": "T"}, {"family": "Miyatake", "given": "Shouta", "initials": "S"}, {"family": "Johansson", "given": "Henrik", "initials": "H"}, {"family": "Hashimoto", "given": "Yasumasa", "initials": "Y"}, {"family": "Nordin", "given": "Joel Z", "initials": "JZ"}, {"family": "Mager", "given": "Imre", "initials": "I"}, {"family": "Aoki", "given": "Misako", "initials": "M"}, {"family": "Graham", "given": "McClorey", "initials": "M"}, {"family": "Sathyaprakash", "given": "Chaitra", "initials": "C"}, {"family": "Roberts", "given": "Thomas C", "initials": "TC"}, {"family": "Wood", "given": "Matthew J A", "initials": "MJA"}, {"family": "Behlke", "given": "Mark A", "initials": "MA"}, {"family": "Andaloussi", "given": "Samir El", "initials": "SE"}], "type": "journal article", "published": "2021-10-13", "journal": {"title": "Front Physiol", "issn": "1664-042X", "volume": "12", "pages": "689179", "issn-l": null}, "abstract": "Splice-switching antisense oligonucleotide- (SSO-) mediated correction of framedisrupting mutation-containing premessenger RNA (mRNA) transcripts using exon skipping is a highly promising treatment method for muscular diseases such as Duchenne muscular dystrophy (DMD). Phosphorothioate (PS) chemistry, a commonly used oligonucleotide modification, has been shown to increase the stability of and improve the pharmacokinetics of SSOs. However, the effect of PS inclusion in 2'-O-methyl SSOs (2OMe) on cellular uptake and splice switching is less well-understood. At present, we demonstrate that the modification of PS facilitates the uptake of 2OMe in H2k-mdx myoblasts. Furthermore, we found a dependency of SSO nuclear accumulation and high splice-switching activity on PS inclusion in 2OMe (2OMePS), as tested in various reporter cell lines carrying pLuc/705. Increased exon-inclusion activity was observed in muscle, neuronal, liver, and bone cell lineages via both the gymnotic uptake and lipofection of 2OMePS. Using the photoactivatable ribonucleoside-enhanced crosslinking and a subsequent proteomic approach, we identified several 2OMePS-binding proteins, which are likely to play a role in the trafficking of 2OMePS to the nucleus. Ablation of one of them, Ncl by small-interfering RNA (siRNA) enhanced 2OMePS uptake in C2C12 myoblasts and upregulated luciferase RNA splicing in the HeLa Luc/705 reporter cell line. Overall, we demonstrate that PS inclusion increases nuclear delivery and splice switching in muscle, neuronal, liver, and bone cell lineages and that the modulation of 2OMePS-binding partners may improve SSO delivery.", "doi": "10.3389/fphys.2021.689179", "pmid": "34721051", "labels": {"Global Proteomics and Proteogenomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC8548633"}], "notes": [], "created": "2022-03-29T10:44:48.421Z", "modified": "2022-03-29T10:44:48.450Z"}], "created": "2022-03-29T10:44:48.437Z", "modified": "2022-03-29T10:44:48.437Z"}