{"entity": "journal", "iuid": "5bb4696e330442eeb55eb3712ab0e35d", "timestamp": "2026-07-12T18:02:04.210Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Front%20Cell%20Neurosci.json"}, "display": {"href": "https://publications.scilifelab.se/journal/Front%20Cell%20Neurosci"}}, "title": "Front Cell Neurosci", "issn": "1662-5102", "issn-l": "1662-5102", "publications_count": 3, "publications": [{"entity": "publication", "iuid": "c6ac69c76dae4296b4cfe9afc374fcaf", "links": {"self": {"href": "https://publications.scilifelab.se/publication/c6ac69c76dae4296b4cfe9afc374fcaf.json"}, "display": {"href": "https://publications.scilifelab.se/publication/c6ac69c76dae4296b4cfe9afc374fcaf"}}, "title": "A Model of Germinal Matrix Hemorrhage in Preterm Rat Pups.", "authors": [{"family": "Jinnai", "given": "Masako", "initials": "M"}, {"family": "Koning", "given": "Gabriella", "initials": "G"}, {"family": "Singh-Mallah", "given": "Gagandeep", "initials": "G"}, {"family": "Jonsdotter", "given": "Andrea", "initials": "A"}, {"family": "Leverin", "given": "Anna-Lena", "initials": "AL"}, {"family": "Svedin", "given": "Pernilla", "initials": "P"}, {"family": "Nair", "given": "Syam", "initials": "S"}, {"family": "Takeda", "given": "Satoru", "initials": "S"}, {"family": "Wang", "given": "Xiaoyang", "initials": "X"}, {"family": "Mallard", "given": "Carina", "initials": "C"}, {"family": "Ek", "given": "Carl Joakim", "initials": "CJ"}, {"family": "Rocha-Ferreira", "given": "Eridan", "initials": "E"}, {"family": "Hagberg", "given": "Henrik", "initials": "H"}], "type": "journal article", "published": "2020-12-03", "journal": {"title": "Front Cell Neurosci", "issn": "1662-5102", "volume": "14", "pages": "535320", "issn-l": "1662-5102"}, "abstract": "Germinal matrix hemorrhage (GMH) is a serious complication in extremely preterm infants associated with neurological deficits and mortality. The purpose of the present study was to develop and characterize a grade III and IV GMH model in postnatal day 5 (P5) rats, the equivalent of preterm human brain maturation. P5 Wistar rats were exposed to unilateral GMH through intracranial injection into the striatum close to the germinal matrix with 0.1, 0.2, or 0.3 U of collagenase VII. During 10 days following GMH induction, motor functions and body weight were assessed and brain tissue collected at P16. Animals were tested for anxiety, motor coordination and motor asymmetry on P22-26 and P36-40. Using immunohistochemical staining and neuropathological scoring we found that a collagenase dose of 0.3 U induced GMH. Neuropathological assessment revealed that the brain injury in the collagenase group was characterized by dilation of the ipsilateral ventricle combined with mild to severe cellular necrosis as well as mild to moderate atrophy at the levels of striatum and subcortical white matter, and to a lesser extent, hippocampus and cortex. Within 0.5 h post-collagenase injection there was clear bleeding at the site of injury, with progressive increase in iron and infiltration of neutrophils in the first 24 h, together with focal microglia activation. By P16, blood was no longer observed, although significant gray and white matter brain infarction persisted. Astrogliosis was also detected at this time-point. Animals exposed to GMH performed worse than controls in the negative geotaxis test and also opened their eyes with latency compared to control animals. At P40, GMH rats spent more time in the center of open field box and moved at higher speed compared to the controls, and continued to show ipsilateral injury in striatum and subcortical white matter. We have established a P5 rat model of collagenase-induced GMH for the study of preterm brain injury. Our results show that P5 rat pups exposed to GMH develop moderate brain injury affecting both gray and white matter associated with delayed eye opening and abnormal motor functions. These animals develop hyperactivity and show reduced anxiety in the juvenile stage.", "doi": "10.3389/fncel.2020.535320", "pmid": "33343300", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC7744792"}], "notes": [], "created": "2023-02-16T08:07:43.072Z", "modified": "2023-02-16T08:07:43.092Z"}, {"entity": "publication", "iuid": "a5d6ff3144ff4fe7960ee2f74f763443", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a5d6ff3144ff4fe7960ee2f74f763443.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a5d6ff3144ff4fe7960ee2f74f763443"}}, "title": "The Interactome of Palmitoyl-Protein Thioesterase 1 (PPT1) Affects Neuronal Morphology and Function.", "authors": [{"family": "Sapir", "given": "Tamar", "initials": "T"}, {"family": "Segal", "given": "Michal", "initials": "M"}, {"family": "Grigoryan", "given": "Gayane", "initials": "G"}, {"family": "Hansson", "given": "Karin M", "initials": "KM"}, {"family": "James", "given": "Peter", "initials": "P"}, {"family": "Segal", "given": "Menahem", "initials": "M"}, {"family": "Reiner", "given": "Orly", "initials": "O"}], "type": "journal article", "published": "2019-03-13", "journal": {"volume": "13", "issn": "1662-5102", "issue": null, "pages": "92", "title": "Front Cell Neurosci", "issn-l": "1662-5102"}, "abstract": "Palmitoyl-protein thioesterase 1 (PPT1) is a depalmitoylation enzyme that is mutated in cases of neuronal ceroid lipofuscinosis (NCL). The hallmarks of the disease include progressive neurodegeneration and blindness, as well as seizures. In the current study, we identified 62 high-confident PPT1-binding proteins. These proteins included a self-interaction of PPT1, two V-type ATPases, calcium voltage-gated channels, cytoskeletal proteins and others. Pathway analysis suggested their involvement in seizures and neuronal morphology. We then proceeded to demonstrate that hippocampal neurons from Ppt1-/- mice exhibit structural deficits, and further investigated electrophysiology parameters in the hippocampi of mutant mice, both in brain slices and dissociated postnatal primary cultures. Our studies reveal new mechanistic features involved in the pathophysiology of this devastating neurodegenerative disease.", "doi": "10.3389/fncel.2019.00092", "pmid": "30918483", "labels": {"Bioinformatics Support, Infrastructure and Training": "Service", "Bioinformatics Support and Infrastructure": "Service", "Bioinformatics (NBIS)": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC6424868"}], "notes": [], "created": "2020-01-07T14:59:07.294Z", "modified": "2021-06-21T13:29:49.685Z"}, {"entity": "publication", "iuid": "7cd520c472f14c8bb1012c1c6299acc1", "links": {"self": {"href": "https://publications.scilifelab.se/publication/7cd520c472f14c8bb1012c1c6299acc1.json"}, "display": {"href": "https://publications.scilifelab.se/publication/7cd520c472f14c8bb1012c1c6299acc1"}}, "title": "Effects of Fluoxetine on Human Embryo Development.", "authors": [{"family": "Kaihola", "given": "Helena", "initials": "H"}, {"family": "Yaldir", "given": "Fatma G", "initials": "FG"}, {"family": "Hreinsson", "given": "Julius", "initials": "J"}, {"family": "H\u00f6rnaeus", "given": "Katarina", "initials": "K"}, {"family": "Bergquist", "given": "Jonas", "initials": "J"}, {"family": "Olivier", "given": "Jocelien D A", "initials": "JD"}, {"family": "\u00c5kerud", "given": "Helena", "initials": "H"}, {"family": "Sundstr\u00f6m-Poromaa", "given": "Inger", "initials": "I"}], "type": "journal article", "published": "2016-06-16", "journal": {"volume": "10", "issn": "1662-5102", "issue": null, "pages": "160", "title": "Front Cell Neurosci", "issn-l": "1662-5102"}, "abstract": "The use of antidepressant treatment during pregnancy is increasing, and selective serotonin reuptake inhibitors (SSRIs) are the most widely prescribed antidepressants in pregnant women. Serotonin plays a role in embryogenesis, and serotonin transporters are expressed in two-cell mouse embryos. Thus, the aim of the present study was to evaluate whether fluoxetine, one of the most prescribed SSRI antidepressant world-wide, exposure influences the timing of different embryo developmental stages, and furthermore, to analyze what protein, and protein networks, are affected by fluoxetine in the early embryo development. Human embryos (n = 48) were randomly assigned to treatment with 0.25 or 0.5 \u03bcM fluoxetine in culture medium. Embryo development was evaluated by time-lapse monitoring. The fluoxetine-induced human embryo proteome was analyzed by shotgun mass spectrometry. Protein secretion from fluoxetine-exposed human embryos was analyzed by use of high-multiplex immunoassay. The lower dose of fluoxetine had no influence on embryo development. A trend toward reduced time between thawing and start of cavitation was noted in embryos treated with 0.5 \u03bcM fluoxetine (p = 0.065). Protein analysis by shotgun mass spectrometry detected 45 proteins that were uniquely expressed in fluoxetine-treated embryos. These proteins are involved in cell growth, survival, proliferation, and inflammatory response. Culturing with 0.5 \u03bcM, but not 0.25 \u03bcM fluoxetine, caused a significant increase in urokinase-type plasminogen activator (uPA) in the culture medium. In conclusion, fluoxetine has marginal effects on the timing of developmental stages in embryos, but induces expression and secretion of several proteins in a manner that depends on dose. For these reasons, and in line with current guidelines, the lowest possible dose of SSRI should be used in pregnant women who need to continue treatment.", "doi": "10.3389/fncel.2016.00160", "pmid": "27378857", "labels": {"Mass Spectrometry-based Proteomics, Uppsala": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC4909759"}], "notes": [], "created": "2017-05-03T13:02:37.755Z", "modified": "2017-06-12T11:40:07.268Z"}], "created": "2017-05-09T09:11:56.457Z", "modified": "2020-11-27T13:14:06.360Z"}