{"entity": "journal", "iuid": "48d23c978d71433cae83d94c26549e89", "timestamp": "2026-07-22T16:52:32.745Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Fish%20Shellfish%20Immunol..json"}, "display": {"href": "https://publications.scilifelab.se/journal/Fish%20Shellfish%20Immunol."}}, "title": "Fish Shellfish Immunol.", "issn": "1095-9947", "issn-l": "1050-4648", "publications_count": 4, "publications": [{"entity": "publication", "iuid": "89dc7c83508f4e3090b61c3c703047f1", "links": {"self": {"href": "https://publications.scilifelab.se/publication/89dc7c83508f4e3090b61c3c703047f1.json"}, "display": {"href": "https://publications.scilifelab.se/publication/89dc7c83508f4e3090b61c3c703047f1"}}, "title": "In Atlantic salmon skin infested with salmon lice, elevated seawater temperatures change gene expression and mucus glycosylation, which promotes pathogen binding.", "authors": [{"family": "Thomsson", "given": "Kristina A", "initials": "KA"}, {"family": "Sveen", "given": "Lene", "initials": "L"}, {"family": "Benktander", "given": "John", "initials": "J"}, {"family": "Dagnachew", "given": "Binyam S", "initials": "BS"}, {"family": "Quintana-Hayashi", "given": "Macarena P", "initials": "MP"}, {"family": "Johansen", "given": "Lill-Heidi", "initials": "LH"}, {"family": "Breiland", "given": "Mette W", "initials": "MW"}, {"family": "Jacq", "given": "Celeste", "initials": "C"}, {"family": "Ytteborg", "given": "Elisabeth", "initials": "E"}, {"family": "Linden", "given": "Sara K", "initials": "SK"}], "type": "journal article", "published": "2025-10-00", "journal": {"title": "Fish Shellfish Immunol.", "issn": "1095-9947", "volume": "165", "pages": "110557", "issn-l": "1050-4648"}, "abstract": "Skin barrier function is paramount for fish health and is likely affected by the predicted increases in seawater temperature. Salmonid skin produces a mucus layer mainly composed of mucins. Mucin glycans regulate interactions with pathogens, including binding to host cells, quorum sensing and regulation of virulence genes. In this work, the objective was to elucidate the Atlantic salmon (Salmo salar) skin mucosal responses to temperature in the presence of salmon lice (Lepeophtheirus salmonis) to mimic salmon louse pressure at sea. A simultaneous louse and temperature challenge trial was performed, at low (5 \u00b0C), medium (10 \u00b0C), and high (17 \u00b0C) temperatures, using a protocol resulting in lice at the same development stage and density in all groups. Temperature affected skin morphology, with a thinner outer epidermal layer with fewer mucous cells at 17 \u00b0C than at 5 \u00b0C. Liquid chromatography-mass spectrometry demonstrated that the skin mucin O-glycome changed with temperature: the most pronounced glycan changes were a decrease of the disaccharide Sialyl-Tn and an increase of the tetrasaccharide NeuAc\u03b12-3Gal\u03b21-3[NeuAc\u03b12-6]GalNAcol and sulfated glycans at 17 \u00b0C. Principal component analysis of gene expression data clustered samples according to temperature treatments, and changes in expression of homologues of human sialyl-, core 1-, Gal, and GalNAc transferase genes were proposed to be linked to the glycan changes observed by mass spectrometry. Finally, Aeromonas salmonicida had a higher ability to bind mucins from fish kept at 17 \u00b0C than at 5 \u00b0C, demonstrating effects of temperature related glycosylation changes on host-pathogen interactions.", "doi": "10.1016/j.fsi.2025.110557", "pmid": "40639705", "labels": {"Glycoproteomics and MS Proteomics": "Collaborative"}, "xrefs": [{"db": "pii", "key": "S1050-4648(25)00446-2"}], "notes": [], "created": "2025-11-20T18:09:00.842Z", "modified": "2025-11-20T18:09:00.847Z"}, {"entity": "publication", "iuid": "cba3159ec5424a728069f17f7d0bdd59", "links": {"self": {"href": "https://publications.scilifelab.se/publication/cba3159ec5424a728069f17f7d0bdd59.json"}, "display": {"href": "https://publications.scilifelab.se/publication/cba3159ec5424a728069f17f7d0bdd59"}}, "title": "The barramundi (Lates calcarifer) mucus glycome is conserved across oral, skin and gill tissues but varies between individuals.", "authors": [{"family": "Thomsson", "given": "Kristina A", "initials": "KA"}, {"family": "Benktander", "given": "John", "initials": "J"}, {"family": "Erhardsson", "given": "Mattias", "initials": "M"}, {"family": "Wynne", "given": "James W", "initials": "JW"}, {"family": "Taylor", "given": "Richard S", "initials": "RS"}, {"family": "Lind\u00e9n", "given": "Sara K", "initials": "SK"}], "type": "journal article", "published": "2025-10-00", "journal": {"title": "Fish Shellfish Immunol.", "issn": "1095-9947", "volume": "165", "pages": "110515", "issn-l": "1050-4648"}, "abstract": "Infections cause recurring setbacks in aquaculture and the mucus covering the fish surfaces is the first barrier pathogens encounter. The mucus is made up by highly glycosylated mucin glycoproteins. The glycan part of the mucins confers many of the mucus properties and pathogen regulating activities of the mucins, but these glycans remain largely uncharacterised in a range of aquacultured fish species. Barramundi (Lates calcarifer), also called Asian Sea Bass, is cultured in southeast Asia, Australia, USA, UK, Netherlands and Israel. Here we identified 74 O-glycans and three N-glycans in mucus from the oral cavity, skin, gills and intestine using liquid chromatography mass spectrometry. O-glycans from the oral cavity, skin and gill mucus were highly acidic and similar between these epithelia. The barramundi O-glycome displayed a relatively large inter-individual variation, which lead to that glycan features differing between oral cavity, skin and gills were not clearly distinguishable, although the intestinal glycan profiles clearly differed from the other epithelial sites. Barramundi intestinal glycans contained large glycans consisting of up to 14 monosaccharides, often including core 2 glycans with diHexNAc epitopes. This glycan library can serve as a platform for other studies, for example aiming for characterising host-microbe interactions, diagnostic purposes or disease intervention therapies. Furthermore, identification of novel glycans adds to the total glycan library available, leading to that artificial intelligence driven glycomics will become more accurate, allowing the glycomics field to move from a manual and time-consuming activity performed by specialists to automatic data analysis, more similar to other omics.", "doi": "10.1016/j.fsi.2025.110515", "pmid": "40578586", "labels": {"Glycoproteomics and MS Proteomics": "Collaborative"}, "xrefs": [{"db": "pii", "key": "S1050-4648(25)00404-8"}], "notes": [], "created": "2025-11-20T18:08:59.622Z", "modified": "2025-11-20T18:08:59.628Z"}, {"entity": "publication", "iuid": "744d8cb1032f4f83a215709024ff1980", "links": {"self": {"href": "https://publications.scilifelab.se/publication/744d8cb1032f4f83a215709024ff1980.json"}, "display": {"href": "https://publications.scilifelab.se/publication/744d8cb1032f4f83a215709024ff1980"}}, "title": "Gilthead seabream mucus glycosylation is complex, differs between epithelial sites and carries unusual poly N-acetylhexosamine motifs.", "authors": [{"family": "Thomsson", "given": "Kristina A", "initials": "KA"}, {"family": "Benktander", "given": "John", "initials": "J"}, {"family": "Toxqui-Rodr\u00edguez", "given": "Socorro", "initials": "S"}, {"family": "Piazzon", "given": "M Carla", "initials": "MC"}, {"family": "Linden", "given": "Sara K", "initials": "SK"}], "type": "journal article", "published": "2024-10-00", "journal": {"title": "Fish Shellfish Immunol.", "issn": "1095-9947", "volume": "153", "pages": "109864", "issn-l": "1050-4648"}, "abstract": "Gilthead seabream (Sparus aurata) is a marine finfish of economic importance in aquaculture. Despite its adaptability to varying culture conditions, gilthead seabream culture can be affected by viral, bacterial or parasitic diseases. The main route of entry of pathogens is through mucosal surfaces. Teleost external and internal surfaces are covered by mucus, mainly comprised of highly glycosylated proteins called mucins. The mucin glycans regulate pathogen growth, adhesion, virulence and inter and intra species communication. Here, we characterized the gilthead seabream mucus glycosylation, compared it to previously described species and investigated associations with microbiota. 214 glycans were identified. The majority of the glycans were found at more than one epithelial surface, but 27, 22 and 89 O-glycan structures were unique to skin, gill and intestinal sample groups, respectively. Six O-glycan core types were observed. The majority of the seabream skin and gill O-glycans were neutral with unusual poly HexNAc motifs. In contrast, seabream intestinal O-glycans were highly acidic and not of the 'poly HexNAc' type observed in skin and gill. Furthermore, gilthead seabream gill mucosa had less oligomannose and more complex N-glycans compared to skin and intestine. The concentration and diversity of bacteria was similar in skin, gill and intestine, but the bacterial species differed between epithelia and co-varied with glycan epitopes. The presence of a complex mucus glycosylation with plenty of glycan epitopes for bacterial foraging, suggest that the skin mucosal defense in seabream includes an abundant resident microbiota. This large library of structures provides a platform for further studies, for example aiming to identifying glycans to use for diagnostic purposes, to study host-microbe interactions or disease intervention therapies.", "doi": "10.1016/j.fsi.2024.109864", "pmid": "39216712", "labels": {"Glycoproteomics and MS Proteomics": "Collaborative"}, "xrefs": [{"db": "pii", "key": "S1050-4648(24)00509-6"}], "notes": [], "created": "2024-11-27T15:30:36.188Z", "modified": "2024-11-27T15:30:50.819Z"}, {"entity": "publication", "iuid": "217dd90bc30e4398bd9961c7966555c2", "links": {"self": {"href": "https://publications.scilifelab.se/publication/217dd90bc30e4398bd9961c7966555c2.json"}, "display": {"href": "https://publications.scilifelab.se/publication/217dd90bc30e4398bd9961c7966555c2"}}, "title": "Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis).", "authors": [{"family": "Hernroth", "given": "B", "initials": "B"}, {"family": "Baden", "given": "S", "initials": "S"}, {"family": "Tassidis", "given": "H", "initials": "H"}, {"family": "H\u00f6rnaeus", "given": "K", "initials": "K"}, {"family": "Guillemant", "given": "J", "initials": "J"}, {"family": "Bergstr\u00f6m Lind", "given": "S", "initials": "S"}, {"family": "Bergquist", "given": "J", "initials": "J"}], "type": "journal article", "published": "2016-08-00", "journal": {"volume": "55", "issn": "1095-9947", "issue": null, "pages": "452-459", "title": "Fish Shellfish Immunol.", "issn-l": "1050-4648"}, "abstract": "Here, we aimed to investigate potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis, as gills are directly facing seawater and the changing pH (predicted to be reduced from \u223c8.1 to \u223c7.7 by 2100). The AMP activity of gill and haemocyte extracts was compared at pH 6.0, 7.7 and 8.1, with a radial diffusion assay against Escherichia coli. The activity of the gill extracts was not affected by pH, while it was significantly reduced with increasing pH in the haemocyte extracts. Gill extracts were also tested against different species of Vibrio (V. parahaemolyticus, V. tubiashii, V. splendidus, V. alginolyticus) at pH 7.7 and 8.1. The metabolic activity of the bacteria decreased by \u223c65-90%, depending on species of bacteria, but was, as in the radial diffusion assay, not affected by pH. The results indicated that AMPs from gills are efficient in a broad pH-range. However, when mussels were pre-exposed for pH 7.7 for four month the gill extracts presented significantly lower inhibit of bacterial growth. A full in-depth proteome investigation of gill extracts, using LC-Orbitrap MS/MS technique, showed that among previously described AMPs from haemocytes of Mytilus, myticin A was found up-regulated in response to lipopolysaccharide, 3\u00a0h post injection. Sporadic occurrence of other immune related peptides/proteins also pointed to a rapid response (0.5-3\u00a0h p.i.). Altogether, our results indicate that the gills of blue mussels constitute an important first line defence adapted to act at the pH of seawater. The antimicrobial activity of the gills is however modulated when mussels are under the pressure of ocean acidification, which may give future advantages for invading pathogens.", "doi": "10.1016/j.fsi.2016.04.007", "pmid": "27288994", "labels": {"Mass Spectrometry-based Proteomics, Uppsala": "Collaborative"}, "xrefs": [{"db": "pii", "key": "S1050-4648(16)30157-7"}], "notes": [], "created": "2017-05-03T13:02:35.353Z", "modified": "2017-06-12T11:40:05.708Z"}], "created": "2017-05-09T09:12:03.853Z", "modified": "2020-11-27T13:14:01.135Z"}