{"entity": "journal", "iuid": "804263301d0047a48ecaa6fc99212cf5", "timestamp": "2026-08-15T06:24:31.238Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Dev.%20Dyn..json"}, "display": {"href": "https://publications.scilifelab.se/journal/Dev.%20Dyn."}}, "title": "Dev. Dyn.", "issn": "1097-0177", "issn-l": "1058-8388", "publications_count": 3, "publications": [{"entity": "publication", "iuid": "ba0afa59df8a4ae1a404a7fdebb00c6a", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ba0afa59df8a4ae1a404a7fdebb00c6a.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ba0afa59df8a4ae1a404a7fdebb00c6a"}}, "title": "The role of Gdf5 in the development of the zebrafish fin endoskeleton.", "authors": [{"family": "Waldmann", "given": "Laura", "initials": "L", "orcid": "0000-0002-3619-0796", "researcher": {"href": "https://publications.scilifelab.se/researcher/360d0e0c567f4bf1ae6967f4dedfad1a.json"}}, {"family": "Leyhr", "given": "Jake", "initials": "J", "orcid": "0000-0003-1815-7818", "researcher": {"href": "https://publications.scilifelab.se/researcher/7a145555c1e44b3f9c8371ba8cf6ad13.json"}}, {"family": "Zhang", "given": "Hanqing", "initials": "H"}, {"family": "Allalou", "given": "Amin", "initials": "A"}, {"family": "\u00d6hman-M\u00e4gi", "given": "Caroline", "initials": "C", "orcid": "0000-0003-2709-9541", "researcher": {"href": "https://publications.scilifelab.se/researcher/1e91cc9f7f5e4e6da24c6f7f9b4437f1.json"}}, {"family": "Haitina", "given": "Tatjana", "initials": "T", "orcid": "0000-0002-8754-5534", "researcher": {"href": "https://publications.scilifelab.se/researcher/ffc0088a2af94e2eb960798d93ec33a5.json"}}], "type": "journal article", "published": "2022-09-00", "journal": {"title": "Dev. Dyn.", "issn": "1097-0177", "volume": "251", "issue": "9", "pages": "1535-1549", "issn-l": "1058-8388"}, "abstract": "The development of the vertebrate limb skeleton requires a complex interaction of multiple factors to facilitate the correct shaping and positioning of bones and joints. Growth and differentiation factor 5 (Gdf5) is involved in patterning appendicular skeletal elements including joints. Expression of gdf5 in zebrafish has been detected in fin mesenchyme condensations and segmentation zones as well as the jaw joint, however, little is known about the functional role of Gdf5 outside of Amniota.\n\nWe generated CRISPR/Cas9 knockout of gdf5 in zebrafish and analyzed the resulting phenotype at different developmental stages. Homozygous gdf5 mutant zebrafish displayed changes in segmentation of the endoskeletal disc and, as a consequence, loss of posterior radials in the pectoral fins. Mutant fish also displayed disorganization and reduced length of endoskeletal elements in the median fins, while joints and mineralization seemed unaffected.\n\nOur study demonstrates the importance of Gdf5 in the development of the zebrafish pectoral and median fin endoskeleton and reveals that the severity of the effect increases from anterior to posterior elements. Our findings are consistent with phenotypes observed in the human and mouse appendicular skeleton in response to Gdf5 knockout, suggesting a broadly conserved role for Gdf5 in Osteichthyes.", "doi": "10.1002/dvdy.399", "pmid": "34242444", "labels": {"Genome Engineering Zebrafish": "Service", "BioImage Informatics": "Collaborative", "Bioinformatics (NBIS)": "Collaborative"}, "xrefs": [], "notes": [], "created": "2021-09-01T10:11:21.795Z", "modified": "2022-12-01T15:54:45.034Z"}, {"entity": "publication", "iuid": "9575d837ecc248a289d22b708e900ad2", "links": {"self": {"href": "https://publications.scilifelab.se/publication/9575d837ecc248a289d22b708e900ad2.json"}, "display": {"href": "https://publications.scilifelab.se/publication/9575d837ecc248a289d22b708e900ad2"}}, "title": "Hypoxia inducible factor-2\u03b1 importance for migration, proliferation, and self-renewal of trunk neural crest cells.", "authors": [{"family": "Niklasson", "given": "Camilla U", "initials": "CU"}, {"family": "Fredlund", "given": "Elina", "initials": "E"}, {"family": "Monni", "given": "Emanuela", "initials": "E"}, {"family": "Lindvall", "given": "Jessica M", "initials": "JM", "orcid": "0000-0002-5042-8481", "researcher": {"href": "https://publications.scilifelab.se/researcher/78debae1bc714b11a97ecf9e9656f1eb.json"}}, {"family": "Kokaia", "given": "Zaal", "initials": "Z"}, {"family": "Hammarlund", "given": "Emma U", "initials": "EU"}, {"family": "Bronner", "given": "Marianne E", "initials": "ME"}, {"family": "Mohlin", "given": "Sofie", "initials": "S", "orcid": "0000-0002-2458-3963", "researcher": {"href": "https://publications.scilifelab.se/researcher/7e095a99e2c84d52b13973c9d46d128a.json"}}], "type": "journal article", "published": "2021-02-00", "journal": {"title": "Dev. Dyn.", "issn": "1097-0177", "volume": "250", "issue": "2", "pages": "191-236", "issn-l": "1058-8388"}, "abstract": "The neural crest is a transient embryonic stem cell population. Hypoxia inducible factor (HIF)-2\u03b1 is associated with neural crest stem cell appearance and aggressiveness in tumors. However, little is known about its role in normal neural crest development.\n\nHere, we show that HIF-2\u03b1 is expressed in trunk neural crest cells of human, murine, and avian embryos. Knockdown as well as overexpression of HIF-2\u03b1 in vivo causes developmental delays, induces proliferation, and self-renewal capacity of neural crest cells while decreasing the proportion of neural crest cells that migrate ventrally to sympathoadrenal sites. Reflecting the in vivo phenotype, transcriptome changes after loss of HIF-2\u03b1 reveal enrichment of genes associated with cancer, invasion, epithelial-to-mesenchymal transition, and growth arrest.\n\nTaken together, these results suggest that expression levels of HIF-2\u03b1 must be strictly controlled during normal trunk neural crest development and that dysregulated levels affects several important features connected to stemness, migration, and development.", "doi": "10.1002/dvdy.253", "pmid": "32940375", "labels": {"Bioinformatics Support, Infrastructure and Training": "Collaborative", "Bioinformatics Support and Infrastructure": "Collaborative", "Clinical Genomics Lund": "Service", "Bioinformatics (NBIS)": "Collaborative", "Clinical Genomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC7891386"}], "notes": [], "created": "2020-11-30T08:12:02.742Z", "modified": "2021-12-02T14:08:45.947Z"}, {"entity": "publication", "iuid": "4cd28ffb0bb040aeb1d18f01821866e5", "links": {"self": {"href": "https://publications.scilifelab.se/publication/4cd28ffb0bb040aeb1d18f01821866e5.json"}, "display": {"href": "https://publications.scilifelab.se/publication/4cd28ffb0bb040aeb1d18f01821866e5"}}, "title": "Expression of chondroitin/dermatan sulfate glycosyltransferases during early zebrafish development.", "authors": [{"family": "Filipek-G\u00f3rniok", "given": "Beata", "initials": "B", "orcid": "0000-0002-6757-5410", "researcher": {"href": "https://publications.scilifelab.se/researcher/11f0b7b3e0b045f082d2aff1dd23ec0e.json"}}, {"family": "Holmborn", "given": "Katarina", "initials": "K"}, {"family": "Haitina", "given": "Tatjana", "initials": "T"}, {"family": "Habicher", "given": "Judith", "initials": "J"}, {"family": "Oliveira", "given": "Marta Bastos", "initials": "MB"}, {"family": "Hellgren", "given": "Charlotte", "initials": "C"}, {"family": "Eriksson", "given": "Inger", "initials": "I"}, {"family": "Kjell\u00e9n", "given": "Lena", "initials": "L"}, {"family": "Kreuger", "given": "Johan", "initials": "J"}, {"family": "Ledin", "given": "Johan", "initials": "J", "orcid": "0000-0002-7319-7735", "researcher": {"href": "https://publications.scilifelab.se/researcher/92e482abc18c49d881d3bf0132b3fbcd.json"}}], "type": "journal article", "published": "2013-08-00", "journal": {"volume": "242", "issn": "1097-0177", "issue": "8", "pages": "964-975", "title": "Dev. Dyn.", "issn-l": "1058-8388"}, "abstract": "Chondroitin/dermatan sulfate (CS/DS) proteoglycans present in the extracellular matrix have important structural and regulatory functions.\n\nSix human genes have previously been shown to catalyze CS/DS polymerization. Here we show that one of these genes, chpf, is represented by two copies in the zebrafish genome, chpfa and chpfb, while the other five human CS/DS glycosyltransferases csgalnact1, csgalnact2, chpf2, chsy1, and chsy3 all have single zebrafish orthologues. The putative zebrafish CS/DS glycosyltransferases are spatially and temporally expressed. Interestingly, overlapping expression of multiple glycosyltransferases coincides with high CS/DS deposition. Finally, whereas the relative levels of the related polysaccharide HS reach steady-state at around 2 days post fertilization, there is a continued relative increase of the CS amounts per larvae during the first 6 days of development, matching the increased cartilage formation.\n\nThere are 7 CS/DS glycosyltransferases in zebrafish, which, based on homology, can be divided into the CSGALNACT, CHSY, and CHPF families. The overlap between intense CS/DS production and the expression of multiple CS/DS glycosyltransferases suggests that efficient CS/DS biosynthesis requires a combination of several glycosyltransferases.", "doi": "10.1002/dvdy.23981", "pmid": "23703795", "labels": {"Genome Engineering Zebrafish": null}, "xrefs": [], "notes": [], "created": "2017-05-04T15:03:43.737Z", "modified": "2021-07-06T16:05:51.247Z"}], "created": "2017-05-09T09:12:25.973Z", "modified": "2020-11-27T13:14:01.257Z"}