{"entity": "journal", "iuid": "6dc7860e8b7d481e88eb7160563c39bc", "timestamp": "2026-06-18T14:21:29.770Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Dev.%20Biol..json"}, "display": {"href": "https://publications.scilifelab.se/journal/Dev.%20Biol."}}, "title": "Dev. Biol.", "issn": "1095-564X", "issn-l": "0012-1606", "publications_count": 2, "publications": [{"entity": "publication", "iuid": "6fdc95562b3e43dd8b114345f285b393", "links": {"self": {"href": "https://publications.scilifelab.se/publication/6fdc95562b3e43dd8b114345f285b393.json"}, "display": {"href": "https://publications.scilifelab.se/publication/6fdc95562b3e43dd8b114345f285b393"}}, "title": "miR-128 regulates non-myocyte hyperplasia, deposition of extracellular matrix and Islet1 expression during newt cardiac regeneration.", "authors": [{"family": "Witman", "given": "Nevin", "initials": "N"}, {"family": "Heigwer", "given": "Jana", "initials": "J"}, {"family": "Thaler", "given": "Barbara", "initials": "B"}, {"family": "Lui", "given": "Weng-Onn", "initials": "WO"}, {"family": "Morrison", "given": "Jamie Ian", "initials": "JI"}], "type": "journal article", "published": "2013-11-15", "journal": {"volume": "383", "issn": "1095-564X", "issue": "2", "pages": "253-263", "title": "Dev. Biol.", "issn-l": "0012-1606"}, "abstract": "Cardiovascular disease is a global scourge to society, with novel therapeutic approaches required in order to alleviate the suffering caused by sustained cardiac damage. MicroRNAs (miRNAs) are being touted as one such approach in the fight against heart disease, acting as possible post-transcriptional molecular triggers responsible for invoking cardiac regeneration. To further ones understanding of miRNAs and cardiac regeneration, it is prudent to learn from organisms that can intrinsically regenerate their hearts following injury. Using the red-spotted newt, an adult chordate capable of cardiac regeneration, we decided to delve deeper into the role miRNAs play during this process. RNA isolated from regenerating newt heart samples, was used in a microarray screen, to identify significantly expressed candidate miRNAs during newt cardiac regeneration. We performed quantitative qPCR analysis on several conserved miRNAs and found one in particular, miR-128, to be significantly elevated when cardiac hyperplasia is at its peak following injury. In-situ hybridisation techniques revealed a localised expression pattern for miR-128 in the cardiomyocytes and non-cardiomyocytes in close proximity to the regeneration zone and in vivo knockdown studies revealed a regulatory role for miR-128 in proliferating non-cardiomyocyte populations and extracellular matrix deposition. Finally, 3'UTR reporter assays revealed Islet1 as a biological target for miR-128, which was confirmed further through in vivo Islet1 transcriptional and translational expression analysis in regenerating newt hearts. From these studies we conclude that miR-128 regulates both cardiac hyperplasia and Islet1 expression during newt heart regeneration and that this information could be translated into future mammalian cardiac studies.", "doi": "10.1016/j.ydbio.2013.09.011", "pmid": "24055866", "labels": {"National Genomics Infrastructure": null, "NGI Stockholm (Genomics Applications)": null, "NGI Stockholm (Genomics Production)": null}, "xrefs": [{"db": "pii", "key": "S0012-1606(13)00485-5"}], "notes": [], "created": "2017-05-04T14:57:51.585Z", "modified": "2020-01-21T13:56:02.925Z"}, {"entity": "publication", "iuid": "457a79d276644f8385739cf57840531b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/457a79d276644f8385739cf57840531b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/457a79d276644f8385739cf57840531b"}}, "title": "Transcriptional profiling of C. elegans DAF-19 uncovers a ciliary base-associated protein and a CDK/CCRK/LF2p-related kinase required for intraflagellar transport.", "authors": [{"family": "Phirke", "given": "Prasad", "initials": "P"}, {"family": "Efimenko", "given": "Evgeni", "initials": "E"}, {"family": "Mohan", "given": "Swetha", "initials": "S"}, {"family": "Burghoorn", "given": "Jan", "initials": "J"}, {"family": "Crona", "given": "Filip", "initials": "F"}, {"family": "Bakhoum", "given": "Mathieu W", "initials": "MW"}, {"family": "Trieb", "given": "Maria", "initials": "M"}, {"family": "Schuske", "given": "Kim", "initials": "K"}, {"family": "Jorgensen", "given": "Erik M", "initials": "EM"}, {"family": "Piasecki", "given": "Brian P", "initials": "BP"}, {"family": "Leroux", "given": "Michel R", "initials": "MR"}, {"family": "Swoboda", "given": "Peter", "initials": "P"}], "type": "journal article", "published": "2011-09-01", "journal": {"volume": "357", "issn": "1095-564X", "issue": "1", "pages": "235-247", "title": "Dev. Biol.", "issn-l": "0012-1606"}, "abstract": "Cilia are ubiquitous cell surface projections that mediate various sensory- and motility-based processes and are implicated in a growing number of multi-organ genetic disorders termed ciliopathies. To identify new components required for cilium biogenesis and function, we sought to further define and validate the transcriptional targets of DAF-19, the ciliogenic C. elegans RFX transcription factor. Transcriptional profiling of daf-19 mutants (which do not form cilia) and wild-type animals was performed using embryos staged to when the cell types developing cilia in the worm, the ciliated sensory neurons (CSNs), still differentiate. Comparisons between the two populations revealed 881 differentially regulated genes with greater than a 1.5-fold increase or decrease in expression. A subset of these was confirmed by quantitative RT-PCR. Transgenic worms expressing transcriptional GFP fusions revealed CSN-specific expression patterns for 11 of 14 candidate genes. We show that two uncharacterized candidate genes, termed dyf-17 and dyf-18 because their corresponding mutants display dye-filling (Dyf) defects, are important for ciliogenesis. DYF-17 localizes at the base of cilia and is specifically required for building the distal segment of sensory cilia. DYF-18 is an evolutionarily conserved CDK7/CCRK/LF2p-related serine/threonine kinase that is necessary for the proper function of intraflagellar transport, a process critical for cilium biogenesis. Together, our microarray study identifies targets of the evolutionarily conserved RFX transcription factor, DAF-19, providing a rich dataset from which to uncover-in addition to DYF-17 and DYF-18-cellular components important for cilium formation and function.", "doi": "10.1016/j.ydbio.2011.06.028", "pmid": "21740898", "labels": {"Bioinformatics and Expression Analysis (BEA)": null}, "xrefs": [{"db": "pii", "key": "S0012-1606(11)01088-8"}, {"db": "pmc", "key": "PMC3888451"}, {"db": "mid", "key": "NIHMS540805"}], "notes": [], "created": "2017-05-04T15:03:09.848Z", "modified": "2017-05-30T12:40:07.051Z"}], "created": "2017-05-09T09:12:30.769Z", "modified": "2020-11-27T13:14:01.056Z"}