{"entity": "journal", "iuid": "0a8753dda6424645972c94f52f299870", "timestamp": "2026-08-15T22:54:24.246Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Cell%20Death%20Discov.json"}, "display": {"href": "https://publications.scilifelab.se/journal/Cell%20Death%20Discov"}}, "title": "Cell Death Discov", "issn": "2058-7716", "issn-l": "2058-7716", "publications_count": 2, "publications": [{"entity": "publication", "iuid": "b5090ee7f17e4311876f2e97125ba47d", "links": {"self": {"href": "https://publications.scilifelab.se/publication/b5090ee7f17e4311876f2e97125ba47d.json"}, "display": {"href": "https://publications.scilifelab.se/publication/b5090ee7f17e4311876f2e97125ba47d"}}, "title": "Cutaneous squamous cell carcinoma-derived extracellular vesicles exert an oncogenic role by activating cancer-associated fibroblasts.", "authors": [{"family": "Li", "given": "Chen", "initials": "C", "orcid": "0000-0002-7199-4298", "researcher": {"href": "https://publications.scilifelab.se/researcher/acadd10dfac94eda9e9c951a8c04d6cb.json"}}, {"family": "Sun", "given": "Chengxi", "initials": "C", "orcid": "0000-0002-1050-5956", "researcher": {"href": "https://publications.scilifelab.se/researcher/8b42a5450dfd4211981fee0603acf321.json"}}, {"family": "Lohcharoenkal", "given": "Warangkana", "initials": "W"}, {"family": "Ali", "given": "Mohamad Moustafa", "initials": "MM", "orcid": "0000-0002-4902-0550", "researcher": {"href": "https://publications.scilifelab.se/researcher/780c944670ff4d7489410895569ac257.json"}}, {"family": "Xing", "given": "Pengwei", "initials": "P"}, {"family": "Zheng", "given": "Wenyi", "initials": "W"}, {"family": "G\u00f6rgens", "given": "Andr\u00e9", "initials": "A", "orcid": "0000-0001-9198-0857", "researcher": {"href": "https://publications.scilifelab.se/researcher/ea5f85e2c23a4515a39a2fa23d665a99.json"}}, {"family": "Gustafsson", "given": "Manuela O", "initials": "MO"}, {"family": "El Andaloussi", "given": "Samir", "initials": "S"}, {"family": "Sonkoly", "given": "Enik\u00f6", "initials": "E"}, {"family": "Pivarcsi", "given": "Andor", "initials": "A", "orcid": "0000-0003-2196-1102", "researcher": {"href": "https://publications.scilifelab.se/researcher/77ca870317234573a3da5dffb24bb268.json"}}], "type": "journal article", "published": "2023-07-26", "journal": {"title": "Cell Death Discov", "issn": "2058-7716", "volume": "9", "issue": "1", "pages": "260", "issn-l": "2058-7716"}, "abstract": "Cutaneous squamous cell carcinoma (cSCC) is a fast-increasing cancer with metastatic potential. Extracellular vesicles (EVs) are small membrane-bound vesicles that play important roles in intercellular communication, particularly in the tumor microenvironment (TME). Here we report that cSCC cells secrete an increased number of EVs relative to normal human epidermal keratinocytes (NHEKs) and that interfering with the capacity of cSCC to secrete EVs inhibits tumor growth in vivo in a xenograft model of human cSCC. Transcriptome analysis of tumor xenografts by RNA-sequencing enabling the simultaneous quantification of both the human and the mouse transcripts revealed that impaired EV-production of cSCC cells prominently altered the phenotype of stromal cells, in particular genes related to extracellular matrix (ECM)-formation and epithelial-mesenchymal transition (EMT). In line with these results, co-culturing of human dermal fibroblasts (HDFs) with cSCC cells, but not with normal keratinocytes in vitro resulted in acquisition of cancer-associated fibroblast (CAF) phenotype. Interestingly, EVs derived from metastatic cSCC cells, but not primary cSCCs or NHEKs, were efficient in converting HDFs to CAFs. Multiplex bead-based flow cytometry assay and mass-spectrometry (MS)-based proteomic analyses revealed the heterogenous cargo of cSCC-derived EVs and that especially EVs derived from metastatic cSCCs carry proteins associated with EV-biogenesis, EMT, and cell migration. Mechanistically, EVs from metastatic cSCC cells result in the activation of TGF\u03b2 signaling in HDFs. Altogether, our study suggests that cSCC-derived EVs mediate cancer-stroma communication, in particular the conversion of fibroblasts to CAFs, which eventually contribute to cSCC progression.", "doi": "10.1038/s41420-023-01555-2", "pmid": "37495566", "labels": {"NGI Short read": "Service", "NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "National Genomics Infrastructure": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10372068"}, {"db": "pii", "key": "10.1038/s41420-023-01555-2"}], "notes": [], "created": "2023-11-29T11:23:30.604Z", "modified": "2024-01-16T13:48:32.858Z"}, {"entity": "publication", "iuid": "a5d51dc3032f481e9020d1483b809ab8", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a5d51dc3032f481e9020d1483b809ab8.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a5d51dc3032f481e9020d1483b809ab8"}}, "title": "BMP signaling is a therapeutic target in ovarian cancer.", "authors": [{"family": "Fukuda", "given": "Tomohiko", "initials": "T"}, {"family": "Fukuda", "given": "Risa", "initials": "R"}, {"family": "Tanabe", "given": "Ryo", "initials": "R"}, {"family": "Koinuma", "given": "Daizo", "initials": "D"}, {"family": "Koyama", "given": "Hiroo", "initials": "H"}, {"family": "Hashizume", "given": "Yoshinobu", "initials": "Y"}, {"family": "Moustakas", "given": "Aristidis", "initials": "A", "orcid": "0000-0001-9131-3827", "researcher": {"href": "https://publications.scilifelab.se/researcher/6c1626d991f3485e81232db174537e6d.json"}}, {"family": "Miyazono", "given": "Kohei", "initials": "K"}, {"family": "Heldin", "given": "Carl-Henrik", "initials": "CH", "orcid": "0000-0002-9508-896X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f705f7c509904a1db721ace2267ca48f.json"}}], "type": "journal article", "published": "2020-12-05", "journal": {"title": "Cell Death Discov", "issn": "2058-7716", "issn-l": "2058-7716", "volume": "6", "issue": "1", "pages": "139"}, "abstract": "BMP signaling has been found to have tumor-promoting as well as tumor-suppressing effects in different types of tumors. In this study, we investigated the effects of BMP signaling and of BMP inhibitors on ovarian cancer (OC) cells in vitro and in vivo. High expression of BMP receptor 2 (BMPR2) correlated with poor overall survival of OC patients in the TCGA dataset. Both BMP2 and BMPR2 enhanced OC cell proliferation, whereas BMP receptor kinase inhibitors inhibited OC cell growth in cell culture as well as in a mouse model. BMP2 also augmented sphere formation, migration, and invasion of OC cells, and induced EMT. High BMP2 expression was observed after chemotherapy of OC patients in the GSE109934 dataset. In accordance, carboplatin, used for the treatment of OC patients, increased BMP2 secretion from OC cells, and induced EMT partially via activation of BMP signaling. Our data suggest that BMP signaling has tumor-promoting effects in OC, and that BMP inhibitors might be useful therapeutic agents for OC patients. Considering that carboplatin treatment augmented BMP2 secretion, the possibility to use a combination of BMP inhibitors and carboplatin in the treatment of OC patients, would be worth exploring.", "doi": "10.1038/s41420-020-00377-w", "pmid": "33298901", "labels": {"National Genomics Infrastructure": "Service", "NGI Uppsala (SNP&SEQ Technology Platform)": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41420-020-00377-w"}, {"db": "pmc", "key": "PMC7719168"}], "notes": [], "created": "2021-01-11T06:31:10.635Z", "modified": "2021-11-10T12:44:15.555Z"}], "created": "2017-05-09T09:11:57.593Z", "modified": "2020-11-27T13:14:09.016Z"}