{"entity": "journal", "iuid": "e297b6a23c6f4248b2bc606703ee36a6", "timestamp": "2026-03-12T20:19:22.797Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Biomaterials.json"}, "display": {"href": "https://publications.scilifelab.se/journal/Biomaterials"}}, "title": "Biomaterials", "issn": "1878-5905", "issn-l": "0142-9612", "publications_count": 2, "publications": [{"entity": "publication", "iuid": "368b06639bb647269ad8da8f78d6f34c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/368b06639bb647269ad8da8f78d6f34c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/368b06639bb647269ad8da8f78d6f34c"}}, "title": "Elastin-like hydrogel stimulates angiogenesis in a severe model of critical limb ischemia (CLI): An insight into the glyco-host response.", "authors": [{"family": "Marsico", "given": "Grazia", "initials": "G"}, {"family": "Jin", "given": "Chunseng", "initials": "C"}, {"family": "Abbah", "given": "Sunny A", "initials": "SA"}, {"family": "Brauchle", "given": "Eva M", "initials": "EM"}, {"family": "Thomas", "given": "Dilip", "initials": "D"}, {"family": "Rebelo", "given": "Ana L\u00facia", "initials": "AL"}, {"family": "Orbani\u0107", "given": "Doriana", "initials": "D"}, {"family": "Chantepie", "given": "Sandrine", "initials": "S"}, {"family": "Contessotto", "given": "Paolo", "initials": "P"}, {"family": "Papy-Garcia", "given": "Dulce", "initials": "D"}, {"family": "Rodriguez-Cabello", "given": "Carlos", "initials": "C"}, {"family": "Kilcoyne", "given": "Michelle", "initials": "M"}, {"family": "Schenke-Layland", "given": "K", "initials": "K"}, {"family": "Karlsson", "given": "N G", "initials": "NG"}, {"family": "McCullagh", "given": "Karl J A", "initials": "KJA"}, {"family": "Pandit", "given": "Abhay", "initials": "A"}], "type": "journal article", "published": "2021-02-00", "journal": {"title": "Biomaterials", "issn": "1878-5905", "volume": "269", "pages": "120641", "issn-l": "0142-9612"}, "abstract": "Critical limb ischemia (CLI) is characterized by the impairment of microcirculation, necrosis and inflammation of the muscular tissue. Although the role of glycans in mediating inflammation has been reported, changes in the glycosylation following muscle ischemia remains poorly understood. Here, a murine CLI model was used to show the increase of high mannose, \u03b1-(2, 6)-sialic acid and the decrease of hybrid and bisected N-glycans as glycosylation associated with the ischemic environment. Using this model, the efficacy of an elastin-like recombinamers (ELR) hydrogel was assessed. The hydrogel modulates key angiogenic signaling pathways, resulting in capillary formation, and ECM remodeling. Arterioles formation, reduction of fibrosis and anti-inflammatory macrophage polarization wa also induced by the hydrogel administration. Modulation of glycosylation was observed, suggesting, in particular, a role for mannosylation and sialylation in the mediation of tissue repair. Our study elucidates the angiogenic potential of the ELR hydrogel for CLI applications and identifies glycosylation alterations as potential new therapeutic targets.", "doi": "10.1016/j.biomaterials.2020.120641", "pmid": "33493768", "labels": {"Glycoproteomics and MS Proteomics": "Service"}, "xrefs": [{"db": "pii", "key": "S0142-9612(20)30888-7"}], "notes": [], "created": "2021-12-09T21:52:59.626Z", "modified": "2024-01-16T13:46:30.866Z"}, {"entity": "publication", "iuid": "ee2faba8752246c29b1bd90de2630ed5", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ee2faba8752246c29b1bd90de2630ed5.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ee2faba8752246c29b1bd90de2630ed5"}}, "title": "Exosomes influence the behavior of human mesenchymal stem cells on titanium surfaces.", "authors": [{"family": "Wang", "given": "Xiaoqin", "initials": "X"}, {"family": "Shah", "given": "Furqan A", "initials": "FA"}, {"family": "Vazirisani", "given": "Forugh", "initials": "F"}, {"family": "Johansson", "given": "Anna", "initials": "A"}, {"family": "Palmquist", "given": "Anders", "initials": "A"}, {"family": "Omar", "given": "Omar", "initials": "O"}, {"family": "Ekstr\u00f6m", "given": "Karin", "initials": "K"}, {"family": "Thomsen", "given": "Peter", "initials": "P"}], "type": "journal article", "published": "2020-02-00", "journal": {"title": "Biomaterials", "issn": "1878-5905", "volume": "230", "issue": null, "pages": "119571", "issn-l": "0142-9612"}, "abstract": "Mesenchymal stem cells (MSCs) have important roles during osseointegration. This study determined (i) if MSC-derived extracellular vesicles (EVs)/exosomes can be immobilized on titanium (Ti) surfaces and influence the behavior of MSCs, (ii) if the response is differentially affected by EVs from expanded vs differentiated MSCs and (iii) if the EV protein cargos predict the functional features of the exosomes. EVs secreted by human adipose-derived MSCs were isolated by ultracentrifugation and analyzed using nanoparticle tracking analysis, Western blotting and relative quantitative mass spectrometry. Fluorescence microscopy, scanning electron microscopy, cell counting assay and quantitative polymerase chain reaction were used to analyze MSC adhesion, proliferation and differentiation. Exosome immobilization on Ti promoted MSC adhesion and spreading after 24 h and proliferation after 3 and 6 days, irrespective of whether the exosomes were obtained from expansion or differentiation conditions. Immobilized exosomes upregulated stromal cell-derived factor (SDF-1\u03b1) gene expression. Cell adhesion molecules and signaling molecules were abundant in the exosomal proteome. The predicted functions of the equally-abundant proteins in both exosome types were in line with the observed biological effects mediated by the exosomes. Thus, exosomes derived from MSCs and immobilized on Ti surfaces interact with MSCs and rapidly promote MSC adhesion and proliferation. These findings provide a novel route for modification of titanium implant surfaces.", "doi": "10.1016/j.biomaterials.2019.119571", "pmid": "31753474", "labels": {"Glycoproteomics and MS Proteomics": "Service"}, "xrefs": [{"db": "pii", "key": "S0142-9612(19)30670-2"}], "notes": [], "created": "2020-01-30T15:58:43.390Z", "modified": "2024-01-16T13:46:30.997Z"}], "created": "2020-01-30T15:58:43.402Z", "modified": "2020-11-27T13:14:07.820Z"}