{"entity": "journal", "iuid": "837c28a1368b410c9095ffa75dd9cdef", "timestamp": "2026-04-16T17:43:49.404Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Acta%20Biomater.json"}, "display": {"href": "https://publications.scilifelab.se/journal/Acta%20Biomater"}}, "title": "Acta Biomater", "issn": "1878-7568", "issn-l": "1742-7061", "publications_count": 4, "publications": [{"entity": "publication", "iuid": "edd85c93e6b64b849fa095b5601afc39", "links": {"self": {"href": "https://publications.scilifelab.se/publication/edd85c93e6b64b849fa095b5601afc39.json"}, "display": {"href": "https://publications.scilifelab.se/publication/edd85c93e6b64b849fa095b5601afc39"}}, "title": "Increased matrix stiffness enhances pro-tumorigenic traits in a physiologically relevant breast tissue- monocyte 3D model.", "authors": [{"family": "Abrahamsson", "given": "Annelie", "initials": "A"}, {"family": "Boroojeni", "given": "Fatemeh Rasti", "initials": "FR"}, {"family": "Naeimipour", "given": "Sajjad", "initials": "S"}, {"family": "Reustle", "given": "Nina", "initials": "N"}, {"family": "Seleg\u00e5rd", "given": "Robert", "initials": "R"}, {"family": "Aili", "given": "Daniel", "initials": "D"}, {"family": "Dabrosin", "given": "Charlotta", "initials": "C"}], "type": "journal article", "published": "2024-04-01", "journal": {"title": "Acta Biomater", "issn": "1878-7568", "volume": "178", "pages": "160-169", "issn-l": "1742-7061"}, "abstract": "High mammographic density, associated with increased tissue stiffness, is a strong risk factor for breast cancer per se. In postmenopausal women there is no differences in the occurrence of ductal carcinoma in situ (DCIS) depending on breast density. Preliminary data suggest that dense breast tissue is associated with a pro-inflammatory microenvironment including infiltrating monocytes. However, the underlying mechanism(s) remains largely unknown. A major roadblock to understanding this risk factor is the lack of relevant in vitro models. A biologically relevant 3D model with tunable stiffness was developed by cross-linking hyaluronic acid. Breast cancer cells were cultured with and without freshly isolated human monocytes. In a unique clinical setting, extracellular proteins were sampled using microdialysis in situ from women with various breast densities. We show that tissue stiffness resembling high mammographic density increases the attachment of monocytes to the cancer cells, increase the expression of adhesion molecules and epithelia-mesenchymal-transition proteins in estrogen receptor (ER) positive breast cancer. Increased tissue stiffness results in increased secretion of similar pro-tumorigenic proteins as those found in human dense breast tissue including inflammatory cytokines, proteases, and growth factors. ER negative breast cancer cells were mostly unaffected suggesting that diverse cancer cell phenotypes may respond differently to tissue stiffness. We introduce a biological relevant model with tunable stiffness that resembles the densities found in normal breast tissue in women. The model will be key for further mechanistic studies. Additionally, our data revealed several pro-tumorigenic pathways that may be exploited for prevention and therapy against breast cancer. STATEMENT OF SIGNIFICANCE: Women with mammographic high-density breasts have a 4-6-fold higher risk of breast cancer than low-density breasts. Biological mechanisms behind this increase are not fully understood and no preventive therapeutics are available. One major reason being a lack of suitable experimental models. Having such models available would greatly enhance the discovery of relevant targets for breast cancer prevention. We present a biologically relevant 3D-model for studies of human dense breasts, providing a platform for investigating both biophysical and biochemical properties that may affect cancer progression. This model will have a major scientific impact on studies for identification of novel targets for breast cancer prevention.", "doi": "10.1016/j.actbio.2024.02.021", "pmid": "38382828", "labels": {"NGI Uppsala (Uppsala Genome Center)": "Service", "National Genomics Infrastructure": "Service"}, "xrefs": [{"db": "pii", "key": "S1742-7061(24)00089-8"}], "notes": [], "created": "2024-11-05T07:23:39.648Z", "modified": "2024-11-05T07:23:39.657Z"}, {"entity": "publication", "iuid": "99480ec8afcc46d399c2d2bf162d97fd", "links": {"self": {"href": "https://publications.scilifelab.se/publication/99480ec8afcc46d399c2d2bf162d97fd.json"}, "display": {"href": "https://publications.scilifelab.se/publication/99480ec8afcc46d399c2d2bf162d97fd"}}, "title": "Zebrafish embryo as a replacement model for initial biocompatibility studies of biomaterials and drug delivery systems.", "authors": [{"family": "Rothenb\u00fccher", "given": "Theresa S P", "initials": "TSP"}, {"family": "Ledin", "given": "Johan", "initials": "J", "orcid": "0000-0002-7319-7735", "researcher": {"href": "https://publications.scilifelab.se/researcher/92e482abc18c49d881d3bf0132b3fbcd.json"}}, {"family": "Gibbs", "given": "David", "initials": "D"}, {"family": "Engqvist", "given": "H\u00e5kan", "initials": "H"}, {"family": "Persson", "given": "Cecilia", "initials": "C", "orcid": "0000-0001-6663-6536", "researcher": {"href": "https://publications.scilifelab.se/researcher/5fb6fe3555374aec90b2a8b9dfd8016b.json"}}, {"family": "Hulsart-Billstr\u00f6m", "given": "Gry", "initials": "G", "orcid": "0000-0003-2422-831X", "researcher": {"href": "https://publications.scilifelab.se/researcher/52fbfff04d284491a9dc55414e7a3240.json"}}], "type": "journal article", "published": "2019-12-00", "journal": {"title": "Acta Biomater", "issn": "1878-7568", "volume": "100", "issue": null, "pages": "235-243", "issn-l": "1742-7061"}, "abstract": "The development of new biomaterials and drug delivery systems necessitates animal experimentation to demonstrate biocompatibility and therapeutic efficacy. Reduction and replacement of the requirement to conduct experiment using full-grown animals has been achieved through utilising zebrafish embryos, a promising bridge model between in vitro and in vivo research. In this review, we consider how zebrafish embryos have been utilised to test both the biocompatibility of materials developed to interact with the human body and drug release studies. Furthermore, we outline the advantages and limitations of this model and review legal and ethical issues. We anticipate increasing application of the zebrafish model for biomaterial evaluation in the near future. STATEMENT OF SIGNIFICANCE: This review aims to evaluate the potential application and suitability of the zebrafish model in the development of biomaterials and drug delivery systems. It creates scientific impact and interest because replacement models are desirable to the society and the scientific community. The continuous development of biomaterials calls for the need to provide solutions for biological testing. This review covers the topic of how the FET model can be applied to evaluate biocompatibility. Further, it explores the zebrafish from the wild-type to the mutant form, followed by a discussion about the ethical considerations and concerns when using the FET model.", "doi": "10.1016/j.actbio.2019.09.038", "pmid": "31585201", "labels": {"Genome Engineering Zebrafish": null}, "xrefs": [{"db": "pii", "key": "S1742-7061(19)30659-2"}], "notes": [], "created": "2021-01-12T09:32:53.682Z", "modified": "2021-07-06T14:27:46.369Z"}, {"entity": "publication", "iuid": "1ad4726d271b4b8dadc7694be18778e6", "links": {"self": {"href": "https://publications.scilifelab.se/publication/1ad4726d271b4b8dadc7694be18778e6.json"}, "display": {"href": "https://publications.scilifelab.se/publication/1ad4726d271b4b8dadc7694be18778e6"}}, "title": "Zebrafish: A possible tool to evaluate bioactive ions.", "authors": [{"family": "Montazerolghaem", "given": "M", "initials": "M"}, {"family": "Nystr\u00f6m", "given": "L", "initials": "L"}, {"family": "Engqvist", "given": "H", "initials": "H"}, {"family": "Karlsson Ott", "given": "M", "initials": "M"}], "type": "journal article", "published": "2015-06-00", "journal": {"volume": "19", "issn": "1878-7568", "issue": null, "pages": "10-14", "title": "Acta Biomater", "issn-l": "1742-7061"}, "abstract": "Zebrafish is a well-established model organism with a skeletal structure that highly resembles mammalian bone. Yet its use in the research field of biomaterials has been limited. One area that could benefit from this model system is the evaluation of ionic dissolution products from different materials. As a proof of concept we have evaluated the effect of silicate ions on the zebrafish larvae and compared it to a well-known osteoblastic cell line, MC3T3-E1 subclone 14. We have shown that sodium metasilicate (125 \u03bcM and 625 \u03bcM) induces more mineralisation in a dose-dependent manner in zebrafish larvae, 9 days post fertilisation as compared to the non-treated group. Moreover the same trends were seen when adding sodium metasilicate to MC3T3-E1 cultures, with more mineralisation and higher ALP levels with higher doses of silicate (25, 125 and 625 \u03bcM). These results indicate the feasibility of zebrafish larvae for ionic dissolution studies. The zebrafish model is superior to isolated cell cultures in the aspect that it includes the whole bone remodelling system, with osteoblasts, osteoclasts and osteocytes. Zebrafish could thus provide a powerful in vivo tool and be a bridge between cell culture systems and mammalian models.", "doi": "10.1016/j.actbio.2015.03.010", "pmid": "25770927", "labels": {"Genome Engineering Zebrafish": null}, "xrefs": [{"db": "pii", "key": "S1742-7061(15)00110-5"}], "notes": [], "created": "2017-05-02T12:58:05.250Z", "modified": "2017-09-06T11:49:54.960Z"}, {"entity": "publication", "iuid": "1e80b05a5795494cac85d4949c7867f6", "links": {"self": {"href": "https://publications.scilifelab.se/publication/1e80b05a5795494cac85d4949c7867f6.json"}, "display": {"href": "https://publications.scilifelab.se/publication/1e80b05a5795494cac85d4949c7867f6"}}, "title": "Scavenging effect of Trolox released from brushite cements.", "authors": [{"family": "Mestres", "given": "Gemma", "initials": "G"}, {"family": "Santos", "given": "Carlos F", "initials": "CF"}, {"family": "Engman", "given": "Lars", "initials": "L"}, {"family": "Persson", "given": "Cecilia", "initials": "C", "orcid": "0000-0001-6663-6536", "researcher": {"href": "https://publications.scilifelab.se/researcher/5fb6fe3555374aec90b2a8b9dfd8016b.json"}}, {"family": "Ott", "given": "Marjam Karlsson", "initials": "MK"}], "type": "journal article", "published": "2015-01-00", "journal": {"volume": "11", "issn": "1878-7568", "issue": null, "pages": "459-466", "title": "Acta Biomater", "issn-l": "1742-7061"}, "abstract": "In this study a brushite cement was doped with the chain-breaking antioxidant Trolox. The effect of the antioxidant on the physical properties of the cement was evaluated and the release of Trolox was monitored by UV spectroscopy. The ability of the Trolox set free to scavenge reactive oxygen species (ROS) released by macrophages was determined in vitro using a luminol-amplified chemiluminescence assay. Trolox did not modify the crystalline phases of the set cement, which mainly formed crystalline brushite after 7 days in humid conditions. The setting time, compressive strength and morphology of the cement also remained unaltered after the addition of the antioxidant. Trolox was slowly released from the cement following a non-Fickian transport mechanism and nearly 64% of the total amount was released after 3 days. Moreover, the capacity of Trolox to scavenge the ROS released by macrophages increased in a dose-dependent manner. Trolox-loaded cements are expected to reduce some of the first harmful effects of acute inflammation and can thus potentially protect the surrounding tissue during implantation of these as well as other materials used in conjunction.", "doi": "10.1016/j.actbio.2014.09.007", "pmid": "25229765", "labels": {"BioMaterial Interactions (BioMat)": null}, "xrefs": [{"db": "pii", "key": "S1742-7061(14)00390-0"}], "notes": [], "created": "2017-05-02T12:57:03.398Z", "modified": "2021-07-06T14:00:31.835Z"}], "created": "2017-05-09T09:12:17.225Z", "modified": "2020-11-27T13:14:07.829Z"}