{"entity": "journal", "iuid": "aae0cf03348240acb47a7c3e43438cf1", "timestamp": "2026-08-11T14:05:06.862Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/ACS%20Sens..json"}, "display": {"href": "https://publications.scilifelab.se/journal/ACS%20Sens."}}, "title": "ACS Sens.", "issn": "2379-3694", "issn-l": "2379-3694", "publications_count": 4, "publications": [{"entity": "publication", "iuid": "e65ee69679804c9db4f4b460585bd4d7", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e65ee69679804c9db4f4b460585bd4d7.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e65ee69679804c9db4f4b460585bd4d7"}}, "title": "Ultra-Sensitive Detection of Bacterial Spores via SERS.", "authors": [{"family": "Segervald", "given": "Jonas", "initials": "J", "orcid": "0009-0002-9248-5748", "researcher": {"href": "https://publications.scilifelab.se/researcher/283c709a8ce74c118e3e1710814fb018.json"}}, {"family": "Malyshev", "given": "Dmitry", "initials": "D", "orcid": "0000-0002-0496-6692", "researcher": {"href": "https://publications.scilifelab.se/researcher/a36e950e3dd143339cf3d246a63ab117.json"}}, {"family": "\u00d6berg", "given": "Rasmus", "initials": "R", "orcid": "0000-0002-0168-0197", "researcher": {"href": "https://publications.scilifelab.se/researcher/d23b2e0213244a598ab42d29c9dbea24.json"}}, {"family": "Z\u00e4ll", "given": "Erik", "initials": "E"}, {"family": "Jia", "given": "Xueen", "initials": "X"}, {"family": "W\u00e5gberg", "given": "Thomas", "initials": "T", "orcid": "0000-0002-5080-8273", "researcher": {"href": "https://publications.scilifelab.se/researcher/1614ac6527654be8b0e15fd079cb4233.json"}}, {"family": "Andersson", "given": "Magnus", "initials": "M", "orcid": "0000-0002-9835-3263", "researcher": {"href": "https://publications.scilifelab.se/researcher/fe860af5d944447086cbf2d00e3beb3e.json"}}], "type": "journal article", "published": "2025-02-28", "journal": {"title": "ACS Sens.", "issn": "2379-3694", "volume": "10", "issue": "2", "pages": "1237-1248", "issn-l": "2379-3694"}, "abstract": "Bacterial spores are highly resilient and capable of surviving extreme conditions, making them a persistent threat in contexts such as disease transmission, food safety, and bioterrorism. Their ability to withstand conventional sterilization methods necessitates rapid and accurate detection techniques to effectively mitigate the risks they present. In this study, we introduce a surface-enhanced Raman spectroscopy (SERS) approach for detecting Bacillus thuringiensis spores by targeting calcium dipicolinate acid (CaDPA), a biomarker uniquely associated with bacterial spores. Our method uses probe sonication to disrupt spores, releasing their CaDPA, which is then detected by SERS on drop-dried supernatant mixed with gold nanorods. This simple approach enables the selective detection of CaDPA, distinguishing it from other spore components and background noise. We demonstrate detection of biogenic CaDPA from concentrations as low as 103 spores/mL, with sensitivity reaching beyond CaDPA levels of a single spore. Finally, we show the method's robustness by detecting CaDPA from a realistic sample of fresh milk mixed with spores. These findings highlight the potential of SERS as a sensitive and specific technique for bacterial spore detection, with implications for fields requiring rapid and reliable spore identification.", "doi": "10.1021/acssensors.4c03151", "pmid": "39847439", "labels": {"Integrated Microscopy Technologies Ume\u00e5": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11877637"}], "notes": [], "created": "2025-01-30T11:37:25.468Z", "modified": "2025-11-03T12:41:02.712Z"}, {"entity": "publication", "iuid": "02cf4d931b3848ceb099beb7b85e0436", "links": {"self": {"href": "https://publications.scilifelab.se/publication/02cf4d931b3848ceb099beb7b85e0436.json"}, "display": {"href": "https://publications.scilifelab.se/publication/02cf4d931b3848ceb099beb7b85e0436"}}, "title": "Investigating New Applications of a Photoswitchable Fluorescent Norbornadiene as a Multifunctional Probe for Delineation of Amyloid Plaque Polymorphism.", "authors": [{"family": "Dreos", "given": "Ambra", "initials": "A", "orcid": "0000-0003-0448-2089", "researcher": {"href": "https://publications.scilifelab.se/researcher/11d65a7542cd443982e123ab00d46e9a.json"}}, {"family": "Ge", "given": "Junyue", "initials": "J"}, {"family": "Najera", "given": "Francisco", "initials": "F", "orcid": "0000-0002-1635-5514", "researcher": {"href": "https://publications.scilifelab.se/researcher/59a60d855d794d45a9fff4746867037f.json"}}, {"family": "Tebikachew", "given": "Behabitu Ergette", "initials": "BE"}, {"family": "Perez-Inestrosa", "given": "Ezequiel", "initials": "E", "orcid": "0000-0001-7546-5273", "researcher": {"href": "https://publications.scilifelab.se/researcher/5ecc62575cbc43d9bfb86efd8299499c.json"}}, {"family": "Moth-Poulsen", "given": "Kasper", "initials": "K", "orcid": "0000-0003-4018-4927", "researcher": {"href": "https://publications.scilifelab.se/researcher/5807cc241ba24cf4a7841086d72dc3b9.json"}}, {"family": "Blennow", "given": "Kaj", "initials": "K"}, {"family": "Zetterberg", "given": "Henrik", "initials": "H"}, {"family": "Hanrieder", "given": "J\u00f6rg", "initials": "J", "orcid": "0000-0001-6059-198X", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e65454100674f98bf8f2575093f2441.json"}}], "type": "journal article", "published": "2023-04-28", "journal": {"title": "ACS Sens.", "issn": "2379-3694", "volume": "8", "issue": "4", "pages": "1500-1509", "issn-l": "2379-3694"}, "abstract": "Amyloid beta (A\u03b2) plaques are a major pathological hallmark of Alzheimer's disease (AD) and constitute of structurally heterogenic entities (polymorphs) that have been implicated in the phenotypic heterogeneity of AD pathology and pathogenesis. Understanding amyloid aggregation has been a critical limiting factor to gain understanding of AD pathogenesis, ultimately reflected in that the underlying mechanism remains elusive. We identified a fluorescent probe in the form of a turn-off photoswitchable norbornadiene derivative (NBD1) with several microenvironment-sensitive properties that make it relevant for applications within advanced fluorescence imaging, for example, multifunctional imaging. We explored the application of NBD1 for in situ delineation of structurally heterogenic A\u03b2 plaques in transgenic AD mouse models. NBD1 plaque imaging shows characteristic broader emission bands in the periphery and more narrow emission bands in the dense cores of mature cored plaques. Further, we demonstrate in situ photoisomerization of NBD1 to quadricyclane and thermal recovery in single plaques, which is relevant for applications within both functional and super-resolution imaging. This is the first time a norbornadiene photoswitch has been used as a probe for fluorescence imaging of A\u03b2 plaque pathology in situ and that its spectroscopic and switching properties have been studied within the specific environment of senile A\u03b2 plaques. These findings open the way toward new applications of NBD-based photoswitchable fluorescent probes for super-resolution or dual-color imaging and multifunctional microscopy of amyloid plaque heterogeneity. This could allow to visualize A\u03b2 plaques with resolution beyond the diffraction limit, label different plaque types, and gain insights into their physicochemical composition.", "doi": "10.1021/acssensors.2c02496", "pmid": "36946692", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10152485"}], "notes": [], "created": "2023-12-01T10:45:15.895Z", "modified": "2023-12-01T10:45:15.961Z"}, {"entity": "publication", "iuid": "7aa9a011dfae4264bb5ed7dbf3b4c135", "links": {"self": {"href": "https://publications.scilifelab.se/publication/7aa9a011dfae4264bb5ed7dbf3b4c135.json"}, "display": {"href": "https://publications.scilifelab.se/publication/7aa9a011dfae4264bb5ed7dbf3b4c135"}}, "title": "Estimating Detection Limits of Potentiometric DNA Sensors Using Surface Plasmon Resonance Analyses.", "authors": [{"family": "Xu", "given": "Xingxing", "initials": "X", "orcid": "0000-0002-1769-4382", "researcher": {"href": "https://publications.scilifelab.se/researcher/c0585b665e994ac5990b4911b0cc5cfa.json"}}, {"family": "Makaraviciute", "given": "Asta", "initials": "A"}, {"family": "Abdurakhmanov", "given": "Eldar", "initials": "E"}, {"family": "Wermeling", "given": "Fredrik", "initials": "F"}, {"family": "Li", "given": "Shiyu", "initials": "S", "orcid": "0000-0003-4948-8353", "researcher": {"href": "https://publications.scilifelab.se/researcher/eaafe0529dbc46fb904b20cb943dad09.json"}}, {"family": "Danielson", "given": "U Helena", "initials": "UH", "orcid": "0000-0003-2728-0340", "researcher": {"href": "https://publications.scilifelab.se/researcher/b2bf7dffedf44237807c23718c72efa6.json"}}, {"family": "Nyholm", "given": "Leif", "initials": "L", "orcid": "0000-0001-9292-016X", "researcher": {"href": "https://publications.scilifelab.se/researcher/b7be3793cb034f04a7303a3ba8d0d4bf.json"}}, {"family": "Zhang", "given": "Zhen", "initials": "Z", "orcid": "0000-0003-4317-9701", "researcher": {"href": "https://publications.scilifelab.se/researcher/b84c807f13484a2abf4ab92ab77e37ec.json"}}], "type": "journal article", "published": "2020-01-24", "journal": {"title": "ACS Sens.", "issn": "2379-3694", "issn-l": "2379-3694", "volume": "5", "issue": "1", "pages": "217-224"}, "abstract": "As the signals of potentiometric-based DNA ion-selective field effect transistor (ISFET) sensors differ largely from report to report, a systematic revisit to this method is needed. Herein, the hybridization of the target and the probe DNA on the sensor surface and its dependence on the surface probe DNA coverage and the ionic strength were systematically investigated by surface plasmon resonance (SPR). The maximum potentiometric DNA hybridization signal that could be registered by an ISFET sensor was estimated based on the SPR measurements, without considering buffering effects from any side interaction on the sensing electrode. We found that under physiological solutions (200 to 300 mM ionic strength), the ISFET sensor could not register the DNA hybridization events on the sensor surface due to Debye screening. Lowering the salt concentration to enlarge the Debye length would at the same time reduce the surface hybridization efficiency, thus suppressing the signal. This adverse effect of low salt concentration on the hybridization efficiency was also found to be more significant on the surface with higher probe coverage due to steric hindrance. With the method of diluting buffer, the maximum potentiometric signal generated by the DNA hybridization was estimated to be only around 120 mV with the lowest detection limit of 30 nM, occurring on a surface with optimized probe coverage and in the tris buffer with 10 mM NaCl. An alternative method would be to achieve high-efficiency hybridization in the buffer with high salt concentration (1 M NaCl) and then to perform potentiometric measurements in the buffer with low salt concentration (1 mM NaCl). Based on the characterization of the stability of the hybridized DNA duplexes on the sensor surface in low salt concentration buffer solutions, the estimated maximum potentiometric signal could be significantly higher using the alternative method. The lowest detection limit for this alternative method was estimated to be around 0.6 nM. This work can serve as an important quantitative reference for potentiometric DNA sensors.", "doi": "10.1021/acssensors.9b02086", "pmid": "31833355", "labels": {"Drug Discovery and Development": "Technology development"}, "xrefs": [], "notes": [], "created": "2020-01-07T13:59:48.906Z", "modified": "2025-10-17T13:05:08.079Z"}, {"entity": "publication", "iuid": "2ffe5135802e43a7b538648d741032d1", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2ffe5135802e43a7b538648d741032d1.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2ffe5135802e43a7b538648d741032d1"}}, "title": "Rapid Newcastle Disease Virus Detection Based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout", "authors": [{"family": "Tian", "given": "Bo", "initials": "B"}, {"family": "Ma", "given": "Jing", "initials": "J"}, {"family": "Zard\u00e1n G\u00f3mez de la Torre", "given": "Teresa", "initials": "T"}, {"family": "B\u00e1lint", "given": "\u00c1d\u00e1m", "initials": "\u00c1"}, {"family": "Donolato", "given": "Marco", "initials": "M"}, {"family": "Hansen", "given": "Mikkel Fougt", "initials": "MF"}, {"family": "Svedlindh", "given": "Peter", "initials": "P"}, {"family": "Str\u00f6mberg", "given": "Mattias", "initials": "M"}], "type": "journal-article", "published": "2016-10-28", "journal": {"volume": "1", "issn": "2379-3694", "issue": "10", "pages": "1228-1234", "title": "ACS Sens.", "issn-l": "2379-3694"}, "abstract": null, "doi": "10.1021/acssensors.6b00379", "pmid": null, "labels": {"BioMaterial Interactions (BioMat)": "Collaborative"}, "xrefs": [], "notes": [], "created": "2017-05-08T07:59:49.843Z", "modified": "2021-06-21T15:54:40.334Z"}], "created": "2017-05-09T09:12:52.493Z", "modified": "2020-11-27T13:14:10.090Z"}