A chromosome-level assembly of the seed beetle Callosobruchus maculatus genome with annotation of its repetitive elements.
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Arnqvist G, Westerberg I, Galbraith J, Sayadi A, Scofield DG, Olsen RA, Immonen E, Bonath F, Ewels P, Suh A
G3 (Bethesda) - (-) - [2023-12-13; online 2023-12-13]
Callosobruchus maculatus is a major agricultural pest of legume crops worldwide and an established model system in ecology and evolution. Yet, current molecular biological resources for this species are limited. Here, we employ Hi-C sequencing to generate a greatly improved genome assembly and we annotate its repetitive elements in a dedicated in-depth effort where we manually curate and classify the most abundant unclassified repeat subfamilies. We present a scaffolded chromosome-level assembly, which is 1.01 Gb in total length with 86% being contained within the 9 autosomes and the X chromosome. Repetitive sequences accounted for 70% of the total assembly. DNA transposons covered 18% of the genome, with the most abundant superfamily being Tc1-Mariner (9.75% of the genome). This new chromosome-level genome assembly of C. maculatus will enable future genetic and evolutionary studies not only of this important species but of beetles more generally.
Bioinformatics Support for Computational Resources [Service]
NGI Stockholm (Genomics Applications) [Collaborative]
NGI Stockholm (Genomics Production) [Service]
National Genomics Infrastructure [Service]
PubMed 38092066
DOI 10.1093/g3journal/jkad266
Crossref 10.1093/g3journal/jkad266
pii: 7471854